含有掺锶银纳米颗粒的明胶水凝胶负载的三维打印钛支架可促进骨细胞分化和抗菌活性,用于骨组织工程。

IF 3.2 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS Biotechnology Journal Pub Date : 2024-08-08 DOI:10.1002/biot.202400288
Ramprasad Anushikaa, S. Shree Ganesh, Venkadesan Sri Swetha Victoria, Abinaya Shanmugavadivu, Krishnaraj Lavanya, Sundaravadhanan Lekhavadhani, Nagarajan Selvamurugan
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引用次数: 0

摘要

骨组织工程克服了传统自体移植物和异体移植物的局限性,为刺激受损组织的再生提供了一种前景广阔的替代方法。最近,钛合金(Ti)植入物在治疗临界尺寸骨缺损方面引起了广泛关注,尤其是随着三维打印技术的发展。虽然钛合金具有令人印象深刻的多功能性,但其缺乏细胞粘附性、成骨性和抗菌性是导致其失败的重要因素。因此,为了克服这些障碍,本研究旨在将诱导骨生成和抗菌线索载荷水凝胶融入三维打印钛(3D-Ti)支架中。研究人员采用直接金属激光烧结法合成了三维钛支架,并在支架中添加了含有掺锶银纳米粒子(Sr-Ag NPs)的明胶(Gel)水凝胶。与银纳米粒子相比,掺锶银纳米粒子能增加骨转录因子 Runx2 mRNA 的表达。我们对生物活性三维杂化支架(3D-Ti/Gel/Sr-Ag NPs)进行了物理化学和材料表征,然后进行了细胞相容性和成骨性评估。含Sr-Ag NPs的支架的微孔和大孔拓扑显示Runx2表达和基质矿化增加,并具有强效抗菌特性。因此,加入了Sr-Ag NP的凝胶水凝胶的三维钛支架有利于成骨细胞的分化和抗菌活性,这表明它们具有骨科应用的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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3D-printed titanium scaffolds loaded with gelatin hydrogel containing strontium-doped silver nanoparticles promote osteoblast differentiation and antibacterial activity for bone tissue engineering

Bone tissue engineering offers a promising alternative to stimulate the regeneration of damaged tissue, overcoming the limitations of conventional autografts and allografts. Recently, titanium alloy (Ti) implants have garnered significant attention for treating critical-sized bone defects, especially with the advancement of 3D printing technology. Although Ti alloys have impressive versatility, their lack of cellular adhesion, osteogenic and antibacterial properties are significant factors that contribute to their failure. Hence, to overcome these obstacles, this study aimed to incorporate osteoinductive and antibacterial cue-loaded hydrogels into 3D-printed Ti (3D-Ti) scaffolds. 3D-Ti scaffolds were synthesized using the direct metal laser sintering method and loaded with a gelatin (Gel) hydrogel containing strontium-doped silver nanoparticles (Sr-Ag NPs). Compared with Ag NPs, Sr-doped Ag NPs increased the expression of Runx2 mRNA, which is a key bone transcription factor. We subjected the bioactive 3D-hybrid scaffolds (3D-Ti/Gel/Sr-Ag NPs) to physicochemical and material characterization, followed by cytocompatibility and osteogenic evaluation. The microporous and macroporous topographies of the scaffolds with Sr-Ag NPs showed increased Runx2 expression and matrix mineralization, with potent antibacterial properties. Therefore, the 3D-Ti scaffolds incorporated with Sr-Ag NP-loaded Gel hydrogels favored osteoblast differentiation and antibacterial activity, indicating their potential for orthopedic applications.

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来源期刊
Biotechnology Journal
Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine
CiteScore
8.90
自引率
2.10%
发文量
123
审稿时长
1.5 months
期刊介绍: Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.
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