Ahmet Uyar, Mustafa Cellat, Özgür Kanat, Muhammed Etyemez, Tuncer Kutlu, Mehmet Zeki Yılmaz Deveci, İlker Yavaş, Müslüm Kuzu
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Tnf-α, Nf-κB levels were significantly higher, and caspase-3 expression was elevated, while PCNA expression decreased. BPAF significantly increased tissue degeneration compared with the control. However, the BPAF + CIN group showed statistically significant improvements in sperm parameters, except for concentration. They also exhibited an increase in testosterone levels and an improvement in MDA and GSH levels compared with the BPAF group. However, GPx activity partially enhanced. Tnf-α and Nf-κB levels were significantly reduced, and caspase-3 levels declined while PCNA and Bcl-2 levels increased. The Johnsen Testicular Biopsy score showed a substantial increase. 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引用次数: 0
摘要
双酚 AF(BPAF)的使用越来越广泛,目前在供人类消费的产品中也发现了双酚 AF。本研究调查了 1,8-蒎烯(CIN)对双酚 AF 引起的生殖毒性的保护作用。实验分为四组,每组八只大鼠:对照组、双酚 AF 组(200 毫克/千克)、CIN 组(200 毫克/千克)和双酚 AF + CIN 组。结果表明,与对照组相比,双酚 AF 组睾酮水平下降,精子参数降低。此外,还观察到 MDA 水平升高,GSH 和 GPx 活性降低。CAT 活性也略有下降。Tnf-α、Nf-κB 水平明显升高,Caspase-3 表达升高,而 PCNA 表达降低。与对照组相比,BPAF 明显增加了组织变性。不过,BPAF + CIN 组的精子参数(除浓度外)在统计学上有明显改善。与双酚 AF 组相比,他们的睾酮水平也有所提高,MDA 和 GSH 水平也有所改善。然而,GPx 活性部分增强。Tnf-α和Nf-κB水平显著降低,Caspase-3水平下降,而PCNA和Bcl-2水平上升。Johnsen睾丸活检评分大幅上升。总之,这些结果表明,CIN 联合治疗可增强大鼠的生殖健康,并对双酚 AF 诱导的睾丸损伤具有抗氧化、抗凋亡和抗炎作用。
Bisphenol AF Caused Reproductive Toxicity in Rats and Cineole Co-Treatment Exhibited Protective Effect.
Bisphenol AF (BPAF) is increasingly used and now found in products intended for human consumption. The protective effect of 1,8-cineole (CIN) against BPAF-induced reproductive toxicity was investigated. Four groups were created, with each group consisting of eight rats: control, BPAF (200 mg/kg), CIN (200 mg/kg), and BPAF + CIN groups. The results demonstrated that the BPAF group exhibited a decline in testosterone levels and a decrease in sperm parameters compared with the control. Additionally, higher levels of MDA were observed, along with lower levels of GSH and GPx activity. CAT activity also decreased slightly. Tnf-α, Nf-κB levels were significantly higher, and caspase-3 expression was elevated, while PCNA expression decreased. BPAF significantly increased tissue degeneration compared with the control. However, the BPAF + CIN group showed statistically significant improvements in sperm parameters, except for concentration. They also exhibited an increase in testosterone levels and an improvement in MDA and GSH levels compared with the BPAF group. However, GPx activity partially enhanced. Tnf-α and Nf-κB levels were significantly reduced, and caspase-3 levels declined while PCNA and Bcl-2 levels increased. The Johnsen Testicular Biopsy score showed a substantial increase. Overall, these results suggest that CIN co-treatment in rats enhanced reproductive health and exhibited antioxidant, antiapoptotic, and anti-inflammatory properties against BPAF-induced testicular damage.
期刊介绍:
Reproductive Sciences (RS) is a peer-reviewed, monthly journal publishing original research and reviews in obstetrics and gynecology. RS is multi-disciplinary and includes research in basic reproductive biology and medicine, maternal-fetal medicine, obstetrics, gynecology, reproductive endocrinology, urogynecology, fertility/infertility, embryology, gynecologic/reproductive oncology, developmental biology, stem cell research, molecular/cellular biology and other related fields.