人参皂苷 Rg1 保护血脑屏障和髓鞘,防止老年小鼠术后认知功能障碍。

IF 1.6 4区 医学 Q4 NEUROSCIENCES Neuroreport Pub Date : 2024-10-02 Epub Date: 2024-07-30 DOI:10.1097/WNR.0000000000002083
Yao Huang, Dianping Yang, Sijing Liao, Xilin Guan, Feiran Zhou, Yan Liu, Yong Wang, Ying Zhang
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引用次数: 0

摘要

本研究建立了术后认知功能障碍(POCD)小鼠模型,以观察炎症、血脑屏障通透性和髓鞘的变化,并探讨人参皂苷Rg1预处理对改善POCD综合征的作用。在异氟烷麻醉下,对15至18个月大的小鼠进行胫骨骨折内固定,建立POCD模型。手术前连续腹腔注射人参皂苷Rg1(40毫克/千克/天)14天。认知功能通过莫里斯水迷宫进行检测。用酶联免疫吸附法检测海马、皮层和血清中白细胞介素-1β和肿瘤坏死因子-α的含量。埃文斯蓝法观察血脑屏障的通透性。定量 PCR 和 Western 印迹法分析了海马中 2',3'-环核苷酸 3'-磷酸二酯酶(CNPase)、髓鞘碱性蛋白(MBP)、β-catenin 和细胞周期蛋白 D1 的 mRNA 水平和蛋白表达水平。蛋白印迹法分析了 ZO-1 和 Wnt1 在海马中的蛋白表达水平。最后,用免疫荧光法检测了 CNPase 和 MBP 在海马中的定位。人参皂苷Rg1能预防老年小鼠的POCD、外周和中枢炎症、血脑屏障渗漏,并能逆转ZO-1、CNPase、MBP和Wnt通路相关分子的下调。临床前研究表明,人参皂苷Rg1通过保护血脑屏障和髓鞘改善了老年小鼠的术后认知功能,其具体机制可能与Wnt/β-catenin通路有关。
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Ginsenoside Rg1 protects the blood-brain barrier and myelin sheath to prevent postoperative cognitive dysfunction in aged mice.

In this study, the postoperative cognitive dysfunction (POCD) mouse model was established to observe the changes in inflammation, blood-brain barrier permeability, and myelin sheath, and we explore the effect of ginsenoside Rg1 pretreatment on improving POCD syndrome. The POCD model of 15- to 18-month-old mice was carried out with internal fixation of tibial fractures under isoflurane anesthesia. Pretreatment was performed by continuous intraperitoneal injection of ginsenoside Rg1(40 mg/kg/day) for 14 days before surgery. The cognitive function was detected by the Morris water maze. The contents of interleukin-1β and tumor necrosis factor-α in the hippocampus, cortex, and serum were detected by ELISA. The permeability of blood-brain barrier was observed by Evans blue. The mRNA levels and protein expression levels of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), myelin basic protein (MBP), beta-catenin, and cyclin D1 in the hippocampus were analyzed by quantitative PCR and western blotting. The protein expression levels of ZO-1 and Wnt1 in the hippocampus were analyzed by western blotting. Finally, the localizations of CNPase and MBP in the hippocampus were detected by immunofluorescence. Ginsenoside Rg1 can prevent POCD, peripheral and central inflammation, and blood-brain barrier leakage, and reverse the downregulation of ZO-1, CNPase, MBP, and Wnt pathway-related molecules in aged mice. Preclinical studies suggest that ginsenoside Rg1 improves postoperative cognitive function in aged mice by protecting the blood-brain barrier and myelin sheath, and its specific mechanism may be related to the Wnt/β-catenin pathway.

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来源期刊
Neuroreport
Neuroreport 医学-神经科学
CiteScore
3.20
自引率
0.00%
发文量
150
审稿时长
1 months
期刊介绍: NeuroReport is a channel for rapid communication of new findings in neuroscience. It is a forum for the publication of short but complete reports of important studies that require very fast publication. Papers are accepted on the basis of the novelty of their finding, on their significance for neuroscience and on a clear need for rapid publication. Preliminary communications are not suitable for the Journal. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool. The core interest of the Journal is on studies that cast light on how the brain (and the whole of the nervous system) works. We aim to give authors a decision on their submission within 2-5 weeks, and all accepted articles appear in the next issue to press.
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