基于纸张的 3D 设备与无电池 NFC 恒电位仪集成,用于胆固醇的非酶检测

Sudkate Chaiyo*, Kanjana Kunpatee, Kurt Kalcher, Abdulhadee Yakoh and Kingkan Pungjunun, 
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引用次数: 0

摘要

胆固醇传感器等便携式电化学分析设备被广泛用于疾病诊断。然而,这些工具体积庞大,而且需要生物受体来特异性检测胆固醇。在此,我们开发了一种新型三维电化学纸基分析装置(3D-ePAD),并将其与近场通信(NFC)恒电位仪相结合,应用于胆固醇的非酶检测。这种 3D-ePAD 平台的设计使所有工作操作都在一个设备上完成,该设备分为折纸 PAD(oPAD)和嵌入式 PAD(iPAD)。固定在 oPAD 上的β-环糊精(β-CD)被用作非酶切检测胆固醇的特异性材料。通过该装置,胆固醇检测与智能手机上的免电池 NFC 电位仪集成在了一起。胆固醇的浓度是通过[Fe(CN)6]3-/4-电流信号作为氧化还原指示剂来检测的,该信号之前已存储在 iPAD 的检测部分中。在最佳条件下,3D-ePAD/NFC 对胆固醇检测的线性检测效率为 1-500 μM,最大检测限为 0.3 μM。此外,该传感器还成功用于测量人体真实血清样本中的胆固醇,结果与商用胆固醇测量仪一致。因此,新的 NFC 操作 3D-ePAD 平台可用作各种生物标记物的非酶定量的替代工具。此外,3D-ePAD/NFC 未来还能支持其他疾病的诊断,因为该设备价格低廉、便于携带、可一次性使用,而且只需少量样本即可运行。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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3D Paper-Based Device Integrated with a Battery-Less NFC Potentiostat for Nonenzymatic Detection of Cholesterol

Portable electrochemical analytical devices such as cholesterol sensors are widely used for disease diagnosis. However, these tools are bulky and require bioreceptors for the specific detection of cholesterol. Herein, a novel 3D electrochemical paper-based analytical device (3D-ePAD) combined with a near-field communication (NFC) potentiostat was developed and applied to the nonenzymatic detection of cholesterol. This 3D-ePAD platform was designed so that all working operations are performed on a single device, which is separated into an origami PAD (oPAD) and an inset PAD (iPAD). β-Cyclodextrin (β-CD), which is immobilized on oPAD, is used as a specific material for the nonenzymatic detection of cholesterol. Through this device, cholesterol detection is integrated with a battery-free NFC potentiostat on a smartphone. The concentration of cholesterol was examined through a [Fe(CN)6]3-/4- current signal as a redox indicator, which was previously stored in the detection part of an iPAD. Under optimal conditions, 3D-ePAD/NFC exhibited a linear detection efficiency of 1–500 μM and a maximum detection limit of 0.3 μM for cholesterol detection. Moreover, the proposed sensor was successfully used to measure cholesterol in real serum samples from humans, and the results were consistent with those of a commercial cholesterol meter. Therefore, the new NFC-operated 3D-ePAD platform can be used as an alternative tool for the nonenzymatic quantification of various biomarkers. In addition, 3D-ePAD/NFC can support the diagnosis of other diseases in the future, as the device is inexpensive, portable, and disposable and functions with low sample volumes.

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来源期刊
ACS Measurement Science Au
ACS Measurement Science Au 化学计量学-
CiteScore
5.20
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0.00%
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0
期刊介绍: ACS Measurement Science Au is an open access journal that publishes experimental computational or theoretical research in all areas of chemical measurement science. Short letters comprehensive articles reviews and perspectives are welcome on topics that report on any phase of analytical operations including sampling measurement and data analysis. This includes:Chemical Reactions and SelectivityChemometrics and Data ProcessingElectrochemistryElemental and Molecular CharacterizationImagingInstrumentationMass SpectrometryMicroscale and Nanoscale systemsOmics (Genomics Proteomics Metabonomics Metabolomics and Bioinformatics)Sensors and Sensing (Biosensors Chemical Sensors Gas Sensors Intracellular Sensors Single-Molecule Sensors Cell Chips Arrays Microfluidic Devices)SeparationsSpectroscopySurface analysisPapers dealing with established methods need to offer a significantly improved original application of the method.
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