用于获得性β-内酰胺酶基因分子检测的 Streck ARM-D® β-内酰胺酶试剂盒性能评估。

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES Journal of global antimicrobial resistance Pub Date : 2024-08-19 DOI:10.1016/j.jgar.2024.08.004
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引用次数: 0

摘要

目的:尽管β-内酰胺酶与临床息息相关,但市场上可用于准确检测β-内酰胺酶的分子工具非常有限。在本研究中,我们评估了 ARM-D® β-内酰胺酶试剂盒的性能,该试剂盒是一种市售的多重 PCR 检测试剂盒,可检测 9 种 β-内酰胺酶基因,包括在美国流行的 5 种主要质粒介导的碳青霉烯酶、ESBL 或 AmpC 基因:从美国疾病控制与预防中心(CDC)和食品药品管理局(FDA)的抗生素耐药菌株库中挑选了113株不同的革兰氏阴性分离菌株,其中42株带有多种β-内酰胺酶,以代表最常检测到的携带质粒介导的β-内酰胺耐药基因的细菌种类:结果:结果与全基因组序列数据进行了比较。在164个β-内酰胺酶基因靶标中,有49个独特的变体,所有靶标都被正确检测到,没有任何交叉反应。灵敏度和特异性分别为 100%(164/164)和 99.9%(852/853):结论:ARM-D® β-内酰胺酶试剂盒能检测出多种β-内酰胺酶基因型,且前期成本较低。Streck 分析法是检测美国公共卫生领域关注的关键 β-内酰胺酶耐药基因的合适而全面的工具。
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Performance evaluation of the Streck ARM-DⓇ Kit, β-Lactamase for molecular detection of acquired β-lactamase genes

Objectives

Despite clinical relevance, commercially available molecular tools for accurate β-lactamase detection are limited. In this study, we evaluated the performance of the ARM-D Kit, β-Lactamase, a commercially available multiplex PCR assay designed to detect nine β-lactamase genes, including the five major plasmid-mediated carbapenemases, ESBL and AmpC genes circulating in the United States.

Methods

A diverse collection of 113 Gram-negative isolates, including 42 with multiple β-lactamases genes, was selected from the U.S. Centers for Disease Control and Prevention (CDC) & Food and Drug Administration (FDA) Antimicrobial Resistance Isolate Bank, to represent the most frequently detected bacterial species carrying plasmid-mediated β-lactam resistance genes.

Results

Results were compared with whole genome sequence data. Of 164 β-lactamase gene targets with 49 unique variants, all were detected correctly without any cross-reactivity. The sensitivity and specificity were 100% (164/164) and 99.9% (852/853), respectively.

Conclusion

The ARM-D Kit, β-Lactamase detected a wide range of β-lactamase genotypes at a low upfront cost. The Streck assay represents a suitable, comprehensive tool for the detection of key β-lactamase resistance genes of public health concern in the United States.

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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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