用于获得性β-内酰胺酶基因分子检测的 Streck ARM-D® β-内酰胺酶试剂盒性能评估。

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES Journal of global antimicrobial resistance Pub Date : 2024-08-19 DOI:10.1016/j.jgar.2024.08.004
Brian B. Yoo , Norihisa Yamamoto , Justina Ilutsik Quintero , Maria Jose Machado , Sarah Sabour , Sara Blosser , Maria Karlsson , James Kamile Rasheed , Allison C. Brown
{"title":"用于获得性β-内酰胺酶基因分子检测的 Streck ARM-D® β-内酰胺酶试剂盒性能评估。","authors":"Brian B. Yoo ,&nbsp;Norihisa Yamamoto ,&nbsp;Justina Ilutsik Quintero ,&nbsp;Maria Jose Machado ,&nbsp;Sarah Sabour ,&nbsp;Sara Blosser ,&nbsp;Maria Karlsson ,&nbsp;James Kamile Rasheed ,&nbsp;Allison C. Brown","doi":"10.1016/j.jgar.2024.08.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>Despite clinical relevance, commercially available molecular tools for accurate β-lactamase detection are limited. In this study, we evaluated the performance of the ARM-D<sup>Ⓡ</sup> Kit, β-Lactamase, a commercially available multiplex PCR assay designed to detect nine β-lactamase genes, including the five major plasmid-mediated carbapenemases, ESBL and AmpC genes circulating in the United States.</p></div><div><h3>Methods</h3><p>A diverse collection of 113 Gram-negative isolates, including 42 with multiple β-lactamases genes, was selected from the U.S. Centers for Disease Control and Prevention (CDC) &amp; Food and Drug Administration (FDA) Antimicrobial Resistance Isolate Bank, to represent the most frequently detected bacterial species carrying plasmid-mediated β-lactam resistance genes.</p></div><div><h3>Results</h3><p>Results were compared with whole genome sequence data. Of 164 β-lactamase gene targets with 49 unique variants, all were detected correctly without any cross-reactivity. The sensitivity and specificity were 100% (164/164) and 99.9% (852/853), respectively.</p></div><div><h3>Conclusion</h3><p>The ARM-D<sup>Ⓡ</sup> Kit, β-Lactamase detected a wide range of β-lactamase genotypes at a low upfront cost. The Streck assay represents a suitable, comprehensive tool for the detection of key β-lactamase resistance genes of public health concern in the United States.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 54-58"},"PeriodicalIF":3.7000,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001577/pdfft?md5=34d94f316abd76d94a0af6246db1a138&pid=1-s2.0-S2213716524001577-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Performance evaluation of the Streck ARM-DⓇ Kit, β-Lactamase for molecular detection of acquired β-lactamase genes\",\"authors\":\"Brian B. Yoo ,&nbsp;Norihisa Yamamoto ,&nbsp;Justina Ilutsik Quintero ,&nbsp;Maria Jose Machado ,&nbsp;Sarah Sabour ,&nbsp;Sara Blosser ,&nbsp;Maria Karlsson ,&nbsp;James Kamile Rasheed ,&nbsp;Allison C. Brown\",\"doi\":\"10.1016/j.jgar.2024.08.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>Despite clinical relevance, commercially available molecular tools for accurate β-lactamase detection are limited. In this study, we evaluated the performance of the ARM-D<sup>Ⓡ</sup> Kit, β-Lactamase, a commercially available multiplex PCR assay designed to detect nine β-lactamase genes, including the five major plasmid-mediated carbapenemases, ESBL and AmpC genes circulating in the United States.</p></div><div><h3>Methods</h3><p>A diverse collection of 113 Gram-negative isolates, including 42 with multiple β-lactamases genes, was selected from the U.S. Centers for Disease Control and Prevention (CDC) &amp; Food and Drug Administration (FDA) Antimicrobial Resistance Isolate Bank, to represent the most frequently detected bacterial species carrying plasmid-mediated β-lactam resistance genes.</p></div><div><h3>Results</h3><p>Results were compared with whole genome sequence data. Of 164 β-lactamase gene targets with 49 unique variants, all were detected correctly without any cross-reactivity. The sensitivity and specificity were 100% (164/164) and 99.9% (852/853), respectively.</p></div><div><h3>Conclusion</h3><p>The ARM-D<sup>Ⓡ</sup> Kit, β-Lactamase detected a wide range of β-lactamase genotypes at a low upfront cost. The Streck assay represents a suitable, comprehensive tool for the detection of key β-lactamase resistance genes of public health concern in the United States.</p></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":\"39 \",\"pages\":\"Pages 54-58\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-08-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001577/pdfft?md5=34d94f316abd76d94a0af6246db1a138&pid=1-s2.0-S2213716524001577-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001577\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524001577","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

摘要

目的:尽管β-内酰胺酶与临床息息相关,但市场上可用于准确检测β-内酰胺酶的分子工具非常有限。在本研究中,我们评估了 ARM-D® β-内酰胺酶试剂盒的性能,该试剂盒是一种市售的多重 PCR 检测试剂盒,可检测 9 种 β-内酰胺酶基因,包括在美国流行的 5 种主要质粒介导的碳青霉烯酶、ESBL 或 AmpC 基因:从美国疾病控制与预防中心(CDC)和食品药品管理局(FDA)的抗生素耐药菌株库中挑选了113株不同的革兰氏阴性分离菌株,其中42株带有多种β-内酰胺酶,以代表最常检测到的携带质粒介导的β-内酰胺耐药基因的细菌种类:结果:结果与全基因组序列数据进行了比较。在164个β-内酰胺酶基因靶标中,有49个独特的变体,所有靶标都被正确检测到,没有任何交叉反应。灵敏度和特异性分别为 100%(164/164)和 99.9%(852/853):结论:ARM-D® β-内酰胺酶试剂盒能检测出多种β-内酰胺酶基因型,且前期成本较低。Streck 分析法是检测美国公共卫生领域关注的关键 β-内酰胺酶耐药基因的合适而全面的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Performance evaluation of the Streck ARM-DⓇ Kit, β-Lactamase for molecular detection of acquired β-lactamase genes

Objectives

Despite clinical relevance, commercially available molecular tools for accurate β-lactamase detection are limited. In this study, we evaluated the performance of the ARM-D Kit, β-Lactamase, a commercially available multiplex PCR assay designed to detect nine β-lactamase genes, including the five major plasmid-mediated carbapenemases, ESBL and AmpC genes circulating in the United States.

Methods

A diverse collection of 113 Gram-negative isolates, including 42 with multiple β-lactamases genes, was selected from the U.S. Centers for Disease Control and Prevention (CDC) & Food and Drug Administration (FDA) Antimicrobial Resistance Isolate Bank, to represent the most frequently detected bacterial species carrying plasmid-mediated β-lactam resistance genes.

Results

Results were compared with whole genome sequence data. Of 164 β-lactamase gene targets with 49 unique variants, all were detected correctly without any cross-reactivity. The sensitivity and specificity were 100% (164/164) and 99.9% (852/853), respectively.

Conclusion

The ARM-D Kit, β-Lactamase detected a wide range of β-lactamase genotypes at a low upfront cost. The Streck assay represents a suitable, comprehensive tool for the detection of key β-lactamase resistance genes of public health concern in the United States.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
期刊最新文献
Surveillance and Characteristics of Vancomycin-Resistant Enterococcus Isolates in a Chinese Tertiary Hospital in Shenzhen, 2018 to 2024. Genomic analysis of IMP-8-producing Enterobacter hormaechei with a novel plasmid pK432-IMP. Monte Carlo simulation for dosage optimization of the best available therapy for bloodstream infections secondary to carbapenemase-producing Klebsiella pneumoniae in critically ill patients. Refining the gut colonization Zophobas morio larvae model using an oral administration of multidrug-resistant Escherichia coli. From Forgotten Cure to Modern Medicine: The Resurgence of Bacteriophage Therapy.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1