番茄叶中类胡萝卜素代谢基因(PSY2、LCYE、CrtRB1 和 NCED1)表达的昼夜节律调控

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Doklady Biochemistry and Biophysics Pub Date : 2024-08-28 DOI:10.1134/S1607672924600611
M. A. Filyushin, A. V. Shchennikova, E. Z. Kochieva
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引用次数: 0

摘要

研究了类胡萝卜素代谢关键基因(PSY2、LCYE、CrtRB1 和 NCED1)在番茄 Solanum lycopersicum L. (栽培品种 Korneevsky)植株光合组织中的昼夜节律表达动态。在一天中的六个时间点对基因表达进行了 qRT-PCR 分析,结果表明 PSY2、LCYE 和 NCED1 的转录物在光照阶段的后半期含量最高,而 CrtRB1 则在暗光阶段的末期含量最高。测定了一天中叶片组织中类胡萝卜素的含量和组成;结果表明,叶片中类胡萝卜素生物合成途径ɛ/β分支化合物的积累量是β/β分支化合物的 1.5 倍。
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Circadian Regulation of Expression of Carotenoid Metabolism Genes (PSY2, LCYE, CrtRB1, and NCED1) in Leaves of Tomato Solanum lycopersicum L.

The circadian dynamics of the expression of key genes of carotenoid metabolism (PSY2, LCYE, CrtRB1, and NCED1) in the photosynthetic tissue of tomato Solanum lycopersicum L. (cultivar Korneevsky) plants was characterized. An in silico analysis of the gene expression pattern was carried out and a high level of their transcripts was detected in the leaf tissue. qRT-PCR analysis of gene expression was performed at six time points during the day and showed the highest levels of PSY2, LCYE, and NCED1 transcripts in the second half of the light phase and CrtRB1 at the end of the dark phase. The content and composition of carotenoids in leaf tissue in the middle of the day was determined; it was shown that the leaf accumulates 1.5 times more compounds of the ɛ/β-branch of carotenoid biosynthesis pathway than compounds of the β/β-branch.

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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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