利用 MIC 测试法,测定秘鲁 ruckeri 耶尔森氏菌分离株对土霉素和氟苯尼考的抗菌敏感性。

IF 1.1 4区 农林科学 Q3 FISHERIES Diseases of aquatic organisms Pub Date : 2024-08-29 DOI:10.3354/dao03810
Marco Medina-Morillo, Rute Irgang, Jefferson Yunis-Aguinaga, Ruben Avendaño-Herrera
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引用次数: 0

摘要

由罗氏耶尔森菌(Yersinia ruckeri)引起的耶尔森菌病已成为秘鲁养殖虹鳟鱼(Oncorhynchus mykiss)最常见的疾病,影响到该国重要的水产养殖区普诺(Puno)和胡宁(Junín)地区。氟苯尼考(FLO)和土霉素(OXY)是最常用的抗菌剂,可减少该病原体造成的损失。本研究分析了 60 株 Y. ruckeri 分离物(普诺和胡宁各 30 株)(包括 CECT 4319T 型菌株、CECT 955 型菌株和 CECT 956 型菌株)对 FLO 和 OXY 的敏感性模式。最低抑菌浓度(MIC)是根据临床和实验室标准研究所发布的标准肉汤稀释法指南确定的。FLO 和 OXY 的 MIC 值分别为 4.0 至 8.0 µg ml-1 和 0.5 至 4.0 µg ml-1。归一化耐药性解释(NRI)分析确定了流行病学临界值,即 FLO ≤16.0 µg ml-1,OXY ≤4.0 µg ml-1。包括采集菌株在内的所有秘鲁分离株对这两种抗菌剂都被归类为野生型。尽管普诺地区对 FLO 的 MIC 值为 8 µg ml-1 的 Y. ruckeri 分离物数量是胡宁地区的两倍多(15 个分离物对 7 个分离物),但 NRI 分析表明,流行病学的临界值同样为 16 µg ml-1;而对 OXY 的临界值,普诺地区为 4.0 µg ml-1,胡宁地区为 2.0 µg ml-1。这项研究为监测秘鲁虹鳟养殖业中新分离出的 Y. ruckeri 对 FLO 和 OXY 的敏感性奠定了基础。
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Antimicrobial susceptibility of Peruvian Yersinia ruckeri isolates to oxytetracycline and florfenicol using MIC testing.

Yersiniosis, caused by Yersinia ruckeri, has become the most common disease in farmed rainbow trout Oncorhynchus mykiss in Peru, affecting Puno and Junín Regions, important aquaculture areas in the country. Florfenicol (FLO) and oxytetracycline (OXY) are the antimicrobials most frequently used to mitigate losses attributed to this pathogen. This study presents an analysis of the susceptibility patterns of 60 Y. ruckeri isolates (30 isolates each from Puno and Junín), including the type strain CECT 4319T and the strains CECT 955 and CECT 956, against FLO and OXY. Minimum inhibitory concentrations (MICs) were determined following the guideline for standard broth dilution method published by the Clinical and Laboratory Standards Institute. MIC results ranged from 4.0 to 8.0 µg ml-1 for FLO and 0.5 to 4.0 µg ml-1 for OXY. Normalized resistance interpretation (NRI) analysis identified epidemiological cut-off values of ≤16.0 µg ml-1 for FLO and ≤4.0 µg ml-1 for OXY. All Peruvian isolates, including the collection strains, were categorized as wild-type for both antimicrobials. Even though the number of Y. ruckeri isolates with MIC values of 8 µg ml-1 for FLO is more than double in Puno than in Junín (15 vs. 7 isolates), the NRI analysis showed the same epidemiological cutoff of 16 µg ml-1; while for OXY, it was 4.0 µg ml-1 for Puno and 2.0 µg ml-1 for Junín. This study establishes the basis for monitoring susceptibility to FLO and OXY in new Y. ruckeri isolates in Peruvian rainbow trout farming.

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来源期刊
Diseases of aquatic organisms
Diseases of aquatic organisms 农林科学-兽医学
CiteScore
3.10
自引率
0.00%
发文量
53
审稿时长
8-16 weeks
期刊介绍: DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically: -Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens -Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)- Diseases due to internal circumstances (innate, idiopathic, genetic)- Diseases due to proliferative disorders (neoplasms)- Disease diagnosis, treatment and prevention- Molecular aspects of diseases- Nutritional disorders- Stress and physical injuries- Epidemiology/epizootiology- Parasitology- Toxicology- Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)- Diseases as indicators of humanity''s detrimental impact on nature- Genomics, proteomics and metabolomics of disease- Immunology and disease prevention- Animal welfare- Zoonosis
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