Guanrui Pan, Chun-Kit Au, Yat-Hing Ham, Jian Zhen Yu, Zongwei Cai, Wan Chan
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引用次数: 0
摘要
评估个人甲醛(FA)暴露量最常用甲酸盐作为生物标志物,因为甲酸盐是 FA 代谢的主要产物。不过,甲酸盐也可能来自其他内源性和外源性物质的代谢或膳食摄入,这可能会导致甲醛暴露的结果被高估。我们开发并验证了一种液相色谱-串联质谱(LC-MS/MS)联用同位素稀释法,用于严格定量分析尿液中的两种主要脂肪酸共轭产物:噻脯氨酸(SPro)和噻脯氨酰甘氨酸(SPro-Gly),这两种产物分别是脂肪酸与内源性半胱氨酸或半胱氨酰甘氨酸反应生成的,可作为评估个人脂肪酸暴露量的标记分子。利用这种新开发的方法,我们测量了吸烟者、化学研究实验室和典型家庭住户以及中国寺庙游客的 FA 暴露水平,其皮尔逊相关系数大于 0.94,表明尿液加合物水平与空气中的 FA 水平之间存在很强的线性相关。尿液中 SPro 和 SPro-Gly 的定量分析可能是评估个人 FA 暴露的一种无创、无干扰的方法。
Urinary Thioproline and Thioprolinyl Glycine as Specific Biomarkers of Formaldehyde Exposure in Humans.
Assessment of personal formaldehyde (FA) exposure is most commonly carried out using formate as a biomarker, as it is the major product from FA metabolism. However, formate could also have originated from the metabolism of other endogenous and exogenous substances or from dietary intake, which may give rise to overestimated results with regard to FA exposure. We have developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with an isotope-dilution method for rigorous quantitation of two major urinary FA conjugation products: thioproline (SPro) and thioprolinyl glycine (SPro-Gly), formed in the reaction between FA and endogenous cysteine or cysteinyl glycine, respectively, as marker molecules to assess personal FA exposure. Using this newly developed method, we measured the FA exposure levels in cigarette smokers, occupants of a chemistry research laboratory and typical domestic household, and visitors to a Chinese temple with a Pearson correlation coefficient greater than 0.94, showing a strong linear correlation between urinary adduct levels and the airborne FA level. It is believed that quantitation of urinary SPro and SPro-Gly may represent a noninvasive, interference-free method for assessing personal FA exposure.
期刊介绍:
Environmental Science & Technology (ES&T) is a co-sponsored academic and technical magazine by the Hubei Provincial Environmental Protection Bureau and the Hubei Provincial Academy of Environmental Sciences.
Environmental Science & Technology (ES&T) holds the status of Chinese core journals, scientific papers source journals of China, Chinese Science Citation Database source journals, and Chinese Academic Journal Comprehensive Evaluation Database source journals. This publication focuses on the academic field of environmental protection, featuring articles related to environmental protection and technical advancements.