Laura Hassall, Daniel A Yara, Rebecca Riches-Duit, Peter Rigsby, Alexandre Dobly, Maxime Vermeulen, Antoine Francotte, Bart Faber, Paul Stickings
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引用次数: 0
摘要
抗原特性、数量和完整性是破伤风疫苗一致性测试中需要评估的关键因素。在此,我们开发了一种 mAb 夹心酶联免疫吸附试验,用于检测人类和动物破伤风疫苗中破伤风类毒素 (TTxd) 的相对数量和质量。该酶联免疫吸附试验具有高度特异性和良好的稀释线性,适用于检测一系列不同产品中的 TTxd。我们使用经过超声处理的非佐剂 TTxd 样品和经过热或氧化压力处理的最终批次疫苗,证明了该检测方法能够区分不同含量的疫苗批次和不同质量的疫苗批次。我们还成功地将该方法转移到了其他实验室,并证明不同的破伤风抗原材料可以作为该方法标准化的参考抗原。结果表明,这种检测方法有可能在不再包括体内效力检测的控制策略中发挥关键作用。
Development of a monoclonal antibody sandwich ELISA for the quality control of human and animal tetanus vaccines.
Antigen identity, quantity and integrity are key factors to be evaluated as part of consistency testing of tetanus vaccines. Here we have developed a monoclonal antibody sandwich ELISA to measure the relative amount and quality of tetanus toxoid (TTxd) in human and animal tetanus vaccines. The ELISA is highly specific, has good dilutional linearity, and is suitable for detecting TTxd in a range of different products. We have demonstrated the ability of the assay to discriminate between batches of different content, using vaccine batches that had been prepared to contain differing amounts of TTxd, and of different quality, using samples of non-adjuvanted TTxd that had been exposed to sonication and final lot vaccines that had been exposed to heat or oxidative stress. We have also demonstrated successful transfer of the method to other laboratories and have shown that different tetanus antigen materials may be able to serve as a reference antigen for standardization of the method. The results show this test has the potential to play a key role in a control strategy no longer including an in vivo potency test.
期刊介绍:
ALTEX publishes original articles, short communications, reviews, as well as news and comments and meeting reports. Manuscripts submitted to ALTEX are evaluated by two expert reviewers. The evaluation takes into account the scientific merit of a manuscript and its contribution to animal welfare and the 3R principle.