Helaine Almeida Queiroz, Lisandra Juvêncio da Silva, Fátima Daiana Dias Barroso, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Érica Rayanne Motta da Costa, Leilson Carvalho de Oliveira, Amanda Dias Barbosa, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira, Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva
{"title":"评估氨氯地平对浮游细胞、发育中生物膜和成熟生物膜中念珠菌菌株的作用。","authors":"Helaine Almeida Queiroz, Lisandra Juvêncio da Silva, Fátima Daiana Dias Barroso, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Érica Rayanne Motta da Costa, Leilson Carvalho de Oliveira, Amanda Dias Barbosa, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira, Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva","doi":"10.1080/17460913.2024.2390286","DOIUrl":null,"url":null,"abstract":"<p><p><b>Aim:</b> To evaluate the antifungal activity of amlodipine against strains of <i>Candida</i> spp. and to its possible mechanism of action.<b>Methods:</b> Broth microdilution tests were used to determine the minimum inhibitory concentration, while the synergistic activity was evaluated by calculating the fractional inhibitory concentration index. The action of amlodipine against biofilms was determined using the MTT assay and its possible mechanism of action was investigated through flow cytometry tests.<b>Results:</b> Amlodipine showed MICs ranging from 62.5 to 250 μg/ml, in addition to action against pre-formed and forming biofilms, with reductions between 50 and 90%. Amlodipine increases the externalization of phosphatidylserine and reduces the cell viability of fungal cells, suggesting apoptosis.<b>Conclusion:</b> Amlodipine had good antifungal activity against planktonic cells and biofilms of <i>Candida</i> spp., by leading the cells to apoptosis.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of amlodipine against strains of <i>Candida</i> spp. in planktonic cells, developing biofilms and mature biofilms.\",\"authors\":\"Helaine Almeida Queiroz, Lisandra Juvêncio da Silva, Fátima Daiana Dias Barroso, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Érica Rayanne Motta da Costa, Leilson Carvalho de Oliveira, Amanda Dias Barbosa, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira, Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva\",\"doi\":\"10.1080/17460913.2024.2390286\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Aim:</b> To evaluate the antifungal activity of amlodipine against strains of <i>Candida</i> spp. and to its possible mechanism of action.<b>Methods:</b> Broth microdilution tests were used to determine the minimum inhibitory concentration, while the synergistic activity was evaluated by calculating the fractional inhibitory concentration index. The action of amlodipine against biofilms was determined using the MTT assay and its possible mechanism of action was investigated through flow cytometry tests.<b>Results:</b> Amlodipine showed MICs ranging from 62.5 to 250 μg/ml, in addition to action against pre-formed and forming biofilms, with reductions between 50 and 90%. Amlodipine increases the externalization of phosphatidylserine and reduces the cell viability of fungal cells, suggesting apoptosis.<b>Conclusion:</b> Amlodipine had good antifungal activity against planktonic cells and biofilms of <i>Candida</i> spp., by leading the cells to apoptosis.</p>\",\"PeriodicalId\":12773,\"journal\":{\"name\":\"Future microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-09-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Future microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/17460913.2024.2390286\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/17460913.2024.2390286","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Evaluation of amlodipine against strains of Candida spp. in planktonic cells, developing biofilms and mature biofilms.
Aim: To evaluate the antifungal activity of amlodipine against strains of Candida spp. and to its possible mechanism of action.Methods: Broth microdilution tests were used to determine the minimum inhibitory concentration, while the synergistic activity was evaluated by calculating the fractional inhibitory concentration index. The action of amlodipine against biofilms was determined using the MTT assay and its possible mechanism of action was investigated through flow cytometry tests.Results: Amlodipine showed MICs ranging from 62.5 to 250 μg/ml, in addition to action against pre-formed and forming biofilms, with reductions between 50 and 90%. Amlodipine increases the externalization of phosphatidylserine and reduces the cell viability of fungal cells, suggesting apoptosis.Conclusion: Amlodipine had good antifungal activity against planktonic cells and biofilms of Candida spp., by leading the cells to apoptosis.
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