来自沙捞越多个西米种植园的西米棕榈转录组数据集。

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES BMC Research Notes Pub Date : 2024-09-05 DOI:10.1186/s13104-024-06924-3
Fifi Hafizzah Pendi, Hasnain Hussain
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引用次数: 0

摘要

目的:西米棕榈(Metroxylon sagu Rottb:西米棕榈(Metroxylon sagu Rottb.)由于西米棕榈生长健壮,沙捞越州政府将其选为该州的商品作物之一,并开设了多个西米棕榈种植园。然而,尽管西米棕榈在种植后的成熟期超过 10 年,但据报道,一些西米棕榈种植园的棕榈树发育不良(不抽干)。针对这一问题的研究已在多个领域展开,但有关分子机制的信息仍然很少。本研究的目的是通过从不同西米棕榈种植园的所有抽干西米棕榈样本中获得叶片转录组,从而确定导致西米棕榈正常表型(抽干)的基因:本研究采用传统的 CTAB 方法从叶片组织中提取总 RNA。转录组测序在 Illumina NovaSeq 6000 平台上进行。使用 DESeq2 软件包进行差异表达分析。在所有三个西米棕榈数据集中,共有 6,119 个差异表达基因,包括 4,384 个下调基因和 1,735 个上调基因。这些数据集有助于深入了解西米棕榈主干中的常见表达基因。
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Transcriptome dataset of Metroxylon sagu palms from multiple sago plantations in Sarawak.

Objective: Sago palm (Metroxylon sagu Rottb.) is one of the most important economic crops abundantly found in Mukah, Sarawak, Malaysia. The robustness of the palm triggered the Sarawak government's selection as one of the state's commodity crops, with the opening of several sago palm plantations. However, stunted (non-trunking) palms were reported in several sago palm plantations despite attaining a maturity period of more than ten years after cultivation. Research targeting this problem has been conducted in various fields, yet information on molecular mechanisms is still scarce. This study aimed to determine the genes responsible for sago palm's normal phenotype (trunking) by attaining leaf transcriptomes from samples of all trunking sago palms from different sago palm plantations.

Data description: The conventional CTAB method was employed in the present investigation to extract total RNA from leaf tissues. Transcriptome sequencing was conducted on the Illumina NovaSeq 6000 platform. Differential expression analysis was performed using the DESeq2 package. A total of 6,119 differentially expressed genes, comprising 4,384 downregulated and 1,735 upregulated genes, were expressed in all three sago palm datasets. The datasets provide insights into the commonly expressed genes among trunking sago palms.

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来源期刊
BMC Research Notes
BMC Research Notes Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.60
自引率
0.00%
发文量
363
审稿时长
15 weeks
期刊介绍: BMC Research Notes publishes scientifically valid research outputs that cannot be considered as full research or methodology articles. We support the research community across all scientific and clinical disciplines by providing an open access forum for sharing data and useful information; this includes, but is not limited to, updates to previous work, additions to established methods, short publications, null results, research proposals and data management plans.
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