木聚糖酶和布氏扁豆乳杆菌对两个成熟阶段收获的玉米青贮的影响

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal Feed Science and Technology Pub Date : 2024-09-06 DOI:10.1016/j.anifeedsci.2024.116111
Wagner Sousa Alves, Alberto Jefferson da Silva Macêdo, Gabriela Duarte Oliveira Leite, Gabriel Ferreira de Lima Cruz, Tâmara Chagas da Silveira, Danielle Nascimento Coutinho, Albert José dos Anjos, Felipe Almeida Soares, Odilon Gomes Pereira, Karina Guimarães Ribeiro
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引用次数: 0

摘要

本研究的目的是评估在两个成熟阶段收获的玉米青贮饲料的有氧稳定性(AS)、发酵特性和营养价值,并评估木聚糖酶是否与布氏扁桃乳杆菌(Lentilactobacillus buchneri)结合使用。在试验 1 中,收获的玉米干物质(DM)含量较低(250 克/千克),采用 4 × 2 因子方案(4 种添加剂和 2 个贮藏期)进行青贮,完全随机设计,4 次重复。测试的添加剂如下:对照组(CT;无接种剂)、布氏酵母菌(LB)、木聚糖酶(XL)和 LB+XL (LBXL)。储存期分别为 30 天和 60 天。贮藏 60 天后,LB 青贮的中性洗涤纤维(P=0.041)和酸性洗涤纤维(P=0.024)含量较低。在 LB 和 LBXL 中,乳酸菌数量较多(P<0.001),而在这些处理中,酵母菌数量减少(P=0.008)。LB 的乳酸浓度在 30 天时较低(P<0.001),在 60 天时较高。枸橼酸处理的乙酸含量在 60 天时更高(P=0.014),但在 30 天时不同处理之间没有差异。LB 和 LBXL 青贮饲料中的 AS 含量更高(P<0.001)。所有添加剂都增加了 DM 的可溶性部分(P=0.015)和有机质(OM;P=0.013)。在所有通过率下,LB 的 DM 和 OM 有效消化率更高(P≤0.001),CT 更低。在试验 2 中,玉米在高 DM 含量(360 克/千克)时收获,采用完全随机设计,5 次重复,使用与试验 1 相同的添加剂进行腐熟。LBXL 青贮饲料的氨含量较低(P=0.043),而 LB 青贮饲料的氨含量较高。LBXL 青贮饲料的木质素(sa)含量较低(P=0.019),而 CT 青贮饲料的木质素(sa)含量较高。丝状真菌(FUN)的数量在 CT 青贮饲料中较高(P=0.029),而在 LBXL 中较低。有氧曝晒 7 天后,丝状真菌的数量也出现了同样的趋势(P=0.018)。在通过率为 2% 时,LBXL 青贮饲料的 DM 和 OM 有效消化率较高(P≤0.029),而 XL 青贮饲料较低。在改善青贮饲料的发酵条件、有氧稳定性和营养价值方面,仅接种布氏乳杆菌比使用木聚糖酶(无论是否与枸橼酸木聚糖酶一起使用)更有效,尤其是在低 DM 含量收获的玉米青贮饲料中。
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Effect of xylanase and Lentilactobacillus buchneri on corn silage harvested at two maturity stages

The aim of this study was to evaluate the aerobic stability (AS), fermentative profile, and nutritive value of corn silage harvested at two maturity stages, with the use of xylanase, associated or not with Lentilactobacillus buchneri. In trial 1, corn was harvested with a low dry matter (DM) content (250 g/kg) and ensiled in a 4 × 2 factorial scheme (4 additives and 2 storage periods), in a completely randomized design, with 4 replications. The additives tested were as follows: control (CT; no inoculant), L. buchneri (LB), xylanase (XL), and LB+XL (LBXL). The storage periods were 30 and 60 days. The neutral detergent fiber (P=0.041) and acid detergent fiber (P=0.024) content were lower in LB silage after 60 days of storage. The lactic acid bacteria population was higher (P<0.001) in LB and LBXL, while the yeast population (P=0.008) decreased in these treatments. The lactic acid concentration was lower (P<0.001) in LB at 30 days and higher at 60 days. The acetic acid content was higher (P=0.014) in LB at 60 days, but there was no difference between treatments at 30 days. The AS was higher (P<0.001) in LB and LBXL silages. All additives increased the soluble fraction of DM (P=0.015) and organic matter (OM; P=0.013). The effective digestibility of DM and OM was higher (P≤0.001) for LB and lower for CT at all passage rates. In trial 2, corn was harvested at a high DM content (360 g/kg) and ensiled in a completely randomized design with 5 replications, using the same additives as in trial 1. The ammonia content was lower (P=0.043) in LBXL silage and higher in LB. The lignin (sa) content was lower (P=0.019) in LBXL silage and higher in CT silage. The population of filamentous fungi (FUN) was higher (P=0.029) in CT silage and lower in LBXL. The same trend was observed in the population of FUN after 7 days of aerobic exposure (P=0.018). The effective digestibility of DM and OM at passage rates of 2 % was higher (P≤0.029) in LBXL silage and lower in XL silage. Inoculating corn silage with only L. buchneri was more efficient in improving the fermentative profile, aerobic stability, and nutritive value of silage than using xylanase associated or not with LB, especially in corn silage harvested at a low DM content.

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来源期刊
Animal Feed Science and Technology
Animal Feed Science and Technology 农林科学-奶制品与动物科学
CiteScore
6.00
自引率
6.20%
发文量
266
审稿时长
3 months
期刊介绍: Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.
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