叠加 Hi-C：为识别单细胞染色体相互作用而提出的解决方案

arXiv - QuanBio - Genomics Pub Date : 2024-08-23 DOI:arxiv-2408.13039

Jia Zhang, Li Xiao, Peng Qi, Yaling Zeng, Xumeng Chen, Duan-fang Liao, Kai Li

{"title":"叠加 Hi-C：为识别单细胞染色体相互作用而提出的解决方案","authors":"Jia Zhang, Li Xiao, Peng Qi, Yaling Zeng, Xumeng Chen, Duan-fang Liao, Kai Li","doi":"arxiv-2408.13039","DOIUrl":null,"url":null,"abstract":"Hi-C sequencing is widely used for analyzing chromosomal interactions. In\nthis study, we propose \"superimposed Hi-C\" which features paired EcoP15I sites\nin a linker to facilitate sticky-end ligation with target DNAs. Superimposed\nHi-C overcomes Hi-C's technical limitations, enabling the identification of\nsingle cell's chromosomal interactions.","PeriodicalId":501070,"journal":{"name":"arXiv - QuanBio - Genomics","volume":"10 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Superimposed Hi-C: A Solution Proposed for Identifying Single Cell's Chromosomal Interactions\",\"authors\":\"Jia Zhang, Li Xiao, Peng Qi, Yaling Zeng, Xumeng Chen, Duan-fang Liao, Kai Li\",\"doi\":\"arxiv-2408.13039\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Hi-C sequencing is widely used for analyzing chromosomal interactions. In\\nthis study, we propose \\\"superimposed Hi-C\\\" which features paired EcoP15I sites\\nin a linker to facilitate sticky-end ligation with target DNAs. Superimposed\\nHi-C overcomes Hi-C's technical limitations, enabling the identification of\\nsingle cell's chromosomal interactions.\",\"PeriodicalId\":501070,\"journal\":{\"name\":\"arXiv - QuanBio - Genomics\",\"volume\":\"10 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"arXiv - QuanBio - Genomics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/arxiv-2408.13039\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"arXiv - QuanBio - Genomics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/arxiv-2408.13039","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

Hi-C 测序被广泛用于分析染色体相互作用。在本研究中，我们提出了 "叠加Hi-C"，其特点是在连接子中加入成对的EcoP15I位点，以促进与目标DNA的粘端连接。叠加Hi-C克服了Hi-C的技术局限性，可鉴定单个细胞的染色体相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Superimposed Hi-C: A Solution Proposed for Identifying Single Cell's Chromosomal Interactions

Hi-C sequencing is widely used for analyzing chromosomal interactions. In this study, we propose "superimposed Hi-C" which features paired EcoP15I sites in a linker to facilitate sticky-end ligation with target DNAs. Superimposed Hi-C overcomes Hi-C's technical limitations, enabling the identification of single cell's chromosomal interactions.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

arXiv - QuanBio - Genomics

自引率

0.00%

发文量