{"title":"乙酰胆碱酯酶抑制作用介导了莽草叶提取物对五带喙库蚊的杀幼虫活性","authors":"Kayeen Vadakkan, Sruthy Satheesan Aravoor, Maya Rajan Mundanttu, Bhavya Krishnamurthy Devanooru, Vidhya Mohanan Puthiyamadathil","doi":"10.1186/s40816-024-00379-6","DOIUrl":null,"url":null,"abstract":"The study was formulated to identify the effect of Mangifera indica leaf extract in inhibiting the growth and metamorphosis of Culex quinquefasciatus larva. Bioassay-guided extraction identified the bioactive fraction, after which GC-MS characterized it. The larvicidal activity was analyzed by administrating extract in various concentrations and then subjecting the mortality rate for probit analysis. The morphological and physiological impact upon larvae was understood by histological analysis and acetylcholinesterase activity assay. The results suggested that the extract possessed a high degree of larvicidal activity, whereas the Dose50 was 225.158 ± 15.168 with a Total Chi-Square of 13.09 and p-value of 0.11. The histological studies revealed notable aberrations among the study subjects compared to the control group due to diminished abdominal tissue integrity. It was also observed that the extract could inhibit the acetylcholinesterase activity, with an LD 50 of 0.9512 µg/ml. The observations made in these studies may be utilized to develop a potential larvicidal agent that could act upon multiple targets.","PeriodicalId":10462,"journal":{"name":"Clinical Phytoscience","volume":"35 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Acetylcholinesterase inhibition mediated the larvicidal activity of Mangifera indica extract against Culex quinquefasciatus\",\"authors\":\"Kayeen Vadakkan, Sruthy Satheesan Aravoor, Maya Rajan Mundanttu, Bhavya Krishnamurthy Devanooru, Vidhya Mohanan Puthiyamadathil\",\"doi\":\"10.1186/s40816-024-00379-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The study was formulated to identify the effect of Mangifera indica leaf extract in inhibiting the growth and metamorphosis of Culex quinquefasciatus larva. Bioassay-guided extraction identified the bioactive fraction, after which GC-MS characterized it. The larvicidal activity was analyzed by administrating extract in various concentrations and then subjecting the mortality rate for probit analysis. The morphological and physiological impact upon larvae was understood by histological analysis and acetylcholinesterase activity assay. The results suggested that the extract possessed a high degree of larvicidal activity, whereas the Dose50 was 225.158 ± 15.168 with a Total Chi-Square of 13.09 and p-value of 0.11. The histological studies revealed notable aberrations among the study subjects compared to the control group due to diminished abdominal tissue integrity. It was also observed that the extract could inhibit the acetylcholinesterase activity, with an LD 50 of 0.9512 µg/ml. The observations made in these studies may be utilized to develop a potential larvicidal agent that could act upon multiple targets.\",\"PeriodicalId\":10462,\"journal\":{\"name\":\"Clinical Phytoscience\",\"volume\":\"35 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical Phytoscience\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s40816-024-00379-6\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Phytoscience","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s40816-024-00379-6","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Acetylcholinesterase inhibition mediated the larvicidal activity of Mangifera indica extract against Culex quinquefasciatus
The study was formulated to identify the effect of Mangifera indica leaf extract in inhibiting the growth and metamorphosis of Culex quinquefasciatus larva. Bioassay-guided extraction identified the bioactive fraction, after which GC-MS characterized it. The larvicidal activity was analyzed by administrating extract in various concentrations and then subjecting the mortality rate for probit analysis. The morphological and physiological impact upon larvae was understood by histological analysis and acetylcholinesterase activity assay. The results suggested that the extract possessed a high degree of larvicidal activity, whereas the Dose50 was 225.158 ± 15.168 with a Total Chi-Square of 13.09 and p-value of 0.11. The histological studies revealed notable aberrations among the study subjects compared to the control group due to diminished abdominal tissue integrity. It was also observed that the extract could inhibit the acetylcholinesterase activity, with an LD 50 of 0.9512 µg/ml. The observations made in these studies may be utilized to develop a potential larvicidal agent that could act upon multiple targets.
期刊介绍：
Clinical Phytoscience is an international, peer-reviewed, interdisciplinary, and open access journal publishing high quality research articles on clinical evidence and use of medicinal plants in the development of efficient and well tolerated phytotherapy. Clinical Phytoscience focuses on phytotherapy, looking at proof of concept, efficacy and safety, to be established “at eye level” compared to pharmacotherapy. The emphasis lies on application oriented topics (efficacy and safety of phytotherapy in a specific indication, including its need and acceptance by the patient). The scientific results published in the journal should contribute to the recovery and maintenance of human health by phytotherapy. Clinical Phytoscience will publish high-quality evidence-based clinical studies and relevant pharmacological studies. Key areas of interest are: -Upper and lower airways, ENT and pneumology -Gynecology -Urology -Nephrology Pediatrics -Intestinal tract -Hepatology -Diabetes/metabolic Syndrome -Immunology and microbiology -Hygiene -Analytics
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