用于莱姆病血清诊断的新型BB0108、BB0126、BB0298、BB0323和BB0689包柔氏菌染色体编码重组蛋白

IF 3.3 3区 医学 Q2 MICROBIOLOGY Pathogens Pub Date : 2024-09-05 DOI:10.3390/pathogens13090767
Weronika Grąźlewska, Tomasz Chmielewski, Beata Fiecek, Lucyna Holec-Gąsior
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引用次数: 0

摘要

从常染色体(s.l.)包虫病(Borrelia burgdorferi sensu lato)中获得了五种染色体编码蛋白 BB0108、BB0126、BB0298、BB0323 和 BB0689,每种蛋白有三个变体,分别代表欧洲最常见的基因种(Borrelia afzelii、Borrelia burgdorferi sensu stricto(s.s.)和 Borrelia garinii)。这些重组蛋白与人血清中的 IgM 和 IgG 抗体的反应性是通过 Western 印迹(WB)和 ELISA 进行评估的。在 IgG-WB 中,蛋白质表现出不同的反应性,BB0108 和 BB0689 的最高反应性约为 40-50%。然而,在 IgM-WB 中,这些蛋白质都没有被特异性抗体识别。基于 BB0108 和 BB0323 三个变体的 IgG-ELISA 的灵敏度分别为 71% 至 82% 和 62% 至 72%。相反,两种检测蛋白的特异性始终高于 82%。利用 BB0323 的单一变体进行的试验在检测 IgM 抗体方面没有任何诊断价值。不过,BB0108 在 52% 至 63% 的受测血清中都能被抗体识别。这些抗原的变体具有一致的反应性,因此看起来很有优势。这一观察结果表明,适当选择在勃氏菌中保存下来的抗原,可以解决欧洲各地血清诊断检测中遇到的灵敏度不一的问题。
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New BB0108, BB0126, BB0298, BB0323, and BB0689 Chromosomally Encoded Recombinant Proteins of Borrelia burgdorferi sensu lato for Serodiagnosis of Lyme Disease
Five chromosomally encoded proteins, BB0108, BB0126, BB0298, BB0323, and BB0689, from Borrelia burgdorferi sensu lato (s.l.), were obtained in three variants each, representing the most common genospecies found in Europe (Borrelia afzelii, Borrelia burgdorferi sensu stricto (s.s.), and Borrelia garinii). The reactivity of these recombinant proteins with the IgM and IgG antibodies present in human serum was assessed using Western blot (WB) and the ELISA. In IgG-WB, the proteins exhibited varying reactivity, peaking at approximately 40–50% for BB0108 and BB0689. However, none of these proteins were recognized by specific antibodies in the IgM-WB. The sensitivity of IgG-ELISA based on three variants of BB0108 and BB0323 ranged from 71% to 82% and from 62% to 72%, respectively. Conversely, the specificity of both tested proteins was consistently above 82%. Tests utilizing single variants of BB0323 did not yield any diagnostic value in detecting IgM antibodies. However, BB0108 demonstrated recognition by antibodies present in 52% to 63% of the tested sera. These antigens appear advantageous due to the consistent reactivity observed across their variants. This observation suggests that appropriate selection of antigens conserved within B. burgdorferi s.l. could offer a solution to the issue of variable sensitivity encountered in serodiagnostic tests across Europe.
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来源期刊
Pathogens
Pathogens Medicine-Immunology and Allergy
CiteScore
6.40
自引率
8.10%
发文量
1285
审稿时长
17.75 days
期刊介绍: Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.
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