{"title":"Morrocan Cynara cardunculus L. var. ferocissima 叶甲醇提取物的植物化学成分含量、抗氧化和抗炎活性","authors":"Habiba Nechchadi, Fatima Ezzahra Kacimi, Armando McDonald, Samira Boulbaroud, Hicham Berrougui, Mhamed Ramchoun","doi":"10.1007/s11130-024-01234-2","DOIUrl":null,"url":null,"abstract":"<p><i>Cyanra cardunclus</i> L. var. <i>ferocissima</i> is widely used in Morocco as a food and in traditional medicine. Therefore, this study aimed to determine, for the first time, the phytochemical content and antioxidant and anti-inflammatory activities of this variety. Qualitative tests were used to screen for the phytochemical compounds present in the extract, and spectrophotometric methods were used for quantification. The sugar profiles were determined using HPLC. Antioxidant activity was determined in vitro using DPPH, FRAP, and total antioxidant activity assays, and anti-inflammatory activity was assessed using serum albumin denaturation and membrane stabilization assays. The extract contained a high amount of total polyphenols, hydrocinnamic acids, anthocyanins, chlorophyll, ortho-diphenols, terpenoids, and triterpenoids. In addition, five sugars were identified with high amounts of raffinose and sucrose. The extract exerted considerable antioxidant activity by scavenging radicals and reducing power. It exerts anti-inflammatory effects by inhibiting protein denaturation and heat-inducing hemolysis. From the correlation results, anthocyanin, polyphenol, and triterpenoid contents were strongly correlated with DPPH free radical scavenging activity. Orthodiphenols, flavonols, and chlorophyll α were strongly correlated with FRAP, whereas orthodiphenols, hydrocinnamic acids, and triterpenoids were strongly correlated with total antioxidant activity. In terms of anti-inflammatory activity, orthodiphenols, hydrocinnamic acids, and triterpenoids correlated strongly with inhibition of bovine serum albumin denaturation activity, whereas terpenoids, flavonols, and chlorophyll correlated strongly with red cell membrane-stabilizing activity. In conclusion, the Moroccan <i>Cynara cardunclus var. ferocissima</i> leaf methanolic extract constitutes a promising source of phytochemicals with considerable antioxidant and anti-inflammatory activity.</p>","PeriodicalId":20092,"journal":{"name":"Plant Foods for Human Nutrition","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phytochemical content, antioxidant, and anti-inflammatory activities of Morrocan Cynara cardunculus L. var. ferocissima leaf methanolic extract\",\"authors\":\"Habiba Nechchadi, Fatima Ezzahra Kacimi, Armando McDonald, Samira Boulbaroud, Hicham Berrougui, Mhamed Ramchoun\",\"doi\":\"10.1007/s11130-024-01234-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Cyanra cardunclus</i> L. var. <i>ferocissima</i> is widely used in Morocco as a food and in traditional medicine. Therefore, this study aimed to determine, for the first time, the phytochemical content and antioxidant and anti-inflammatory activities of this variety. Qualitative tests were used to screen for the phytochemical compounds present in the extract, and spectrophotometric methods were used for quantification. The sugar profiles were determined using HPLC. Antioxidant activity was determined in vitro using DPPH, FRAP, and total antioxidant activity assays, and anti-inflammatory activity was assessed using serum albumin denaturation and membrane stabilization assays. The extract contained a high amount of total polyphenols, hydrocinnamic acids, anthocyanins, chlorophyll, ortho-diphenols, terpenoids, and triterpenoids. In addition, five sugars were identified with high amounts of raffinose and sucrose. The extract exerted considerable antioxidant activity by scavenging radicals and reducing power. It exerts anti-inflammatory effects by inhibiting protein denaturation and heat-inducing hemolysis. From the correlation results, anthocyanin, polyphenol, and triterpenoid contents were strongly correlated with DPPH free radical scavenging activity. Orthodiphenols, flavonols, and chlorophyll α were strongly correlated with FRAP, whereas orthodiphenols, hydrocinnamic acids, and triterpenoids were strongly correlated with total antioxidant activity. In terms of anti-inflammatory activity, orthodiphenols, hydrocinnamic acids, and triterpenoids correlated strongly with inhibition of bovine serum albumin denaturation activity, whereas terpenoids, flavonols, and chlorophyll correlated strongly with red cell membrane-stabilizing activity. In conclusion, the Moroccan <i>Cynara cardunclus var. ferocissima</i> leaf methanolic extract constitutes a promising source of phytochemicals with considerable antioxidant and anti-inflammatory activity.</p>\",\"PeriodicalId\":20092,\"journal\":{\"name\":\"Plant Foods for Human Nutrition\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2024-09-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Foods for Human Nutrition\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11130-024-01234-2\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Foods for Human Nutrition","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11130-024-01234-2","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
摘要
Cyanra cardunclus L. var. ferocissima 在摩洛哥被广泛用作食品和传统药物。因此,本研究旨在首次确定该品种的植物化学成分含量以及抗氧化和抗炎活性。研究采用定性测试筛选提取物中的植物化学成分,并使用分光光度法进行定量。采用高效液相色谱法测定了糖的概况。抗氧化活性采用 DPPH、FRAP 和总抗氧化活性测定法进行体外测定,抗炎活性采用血清白蛋白变性和膜稳定测定法进行评估。提取物中含有大量的总多酚、氢化肉桂酸、花青素、叶绿素、正二酚、萜类和三萜类化合物。此外,还发现了五种糖类,其中棉子糖和蔗糖含量较高。该提取物通过清除自由基和还原力发挥了相当大的抗氧化活性。它通过抑制蛋白质变性和热引起的溶血而发挥抗炎作用。从相关性结果来看,花青素、多酚和三萜含量与 DPPH 自由基清除活性密切相关。正二萜、黄酮醇和叶绿素α与 FRAP 密切相关,而正二萜、氢化肉桂酸和三萜类化合物与总抗氧化活性密切相关。在抗炎活性方面,正二萜、氢化肉桂酸和三萜类化合物与抑制牛血清白蛋白变性活性密切相关,而萜类化合物、黄酮醇和叶绿素与红细胞膜稳定活性密切相关。总之,摩洛哥 Cynara cardunclus 变种 ferocissima 叶甲醇提取物是一种很有前景的植物化学物质来源,具有相当强的抗氧化和抗炎活性。
Phytochemical content, antioxidant, and anti-inflammatory activities of Morrocan Cynara cardunculus L. var. ferocissima leaf methanolic extract
Cyanra cardunclus L. var. ferocissima is widely used in Morocco as a food and in traditional medicine. Therefore, this study aimed to determine, for the first time, the phytochemical content and antioxidant and anti-inflammatory activities of this variety. Qualitative tests were used to screen for the phytochemical compounds present in the extract, and spectrophotometric methods were used for quantification. The sugar profiles were determined using HPLC. Antioxidant activity was determined in vitro using DPPH, FRAP, and total antioxidant activity assays, and anti-inflammatory activity was assessed using serum albumin denaturation and membrane stabilization assays. The extract contained a high amount of total polyphenols, hydrocinnamic acids, anthocyanins, chlorophyll, ortho-diphenols, terpenoids, and triterpenoids. In addition, five sugars were identified with high amounts of raffinose and sucrose. The extract exerted considerable antioxidant activity by scavenging radicals and reducing power. It exerts anti-inflammatory effects by inhibiting protein denaturation and heat-inducing hemolysis. From the correlation results, anthocyanin, polyphenol, and triterpenoid contents were strongly correlated with DPPH free radical scavenging activity. Orthodiphenols, flavonols, and chlorophyll α were strongly correlated with FRAP, whereas orthodiphenols, hydrocinnamic acids, and triterpenoids were strongly correlated with total antioxidant activity. In terms of anti-inflammatory activity, orthodiphenols, hydrocinnamic acids, and triterpenoids correlated strongly with inhibition of bovine serum albumin denaturation activity, whereas terpenoids, flavonols, and chlorophyll correlated strongly with red cell membrane-stabilizing activity. In conclusion, the Moroccan Cynara cardunclus var. ferocissima leaf methanolic extract constitutes a promising source of phytochemicals with considerable antioxidant and anti-inflammatory activity.
期刊介绍:
Plant Foods for Human Nutrition (previously Qualitas Plantarum) is an international journal that publishes reports of original research and critical reviews concerned with the improvement and evaluation of the nutritional quality of plant foods for humans, as they are influenced by:
- Biotechnology (all fields, including molecular biology and genetic engineering)
- Food science and technology
- Functional, nutraceutical or pharma foods
- Other nutrients and non-nutrients inherent in plant foods