{"title":"两阶段播种策略及其多反应优化,提高尼泊尔德巴里酵母 NCYC 3413 的木糖醇产量","authors":"","doi":"10.1016/j.biortech.2024.131469","DOIUrl":null,"url":null,"abstract":"<div><p>The aim was to develop a two-stage seeding strategy and its optimization to enhance the conversion of xylose to xylitol by <em>Debaryomyces nepalensis</em> NCYC 3413. To develop efficient seed, multi-response optimization was employed to obtain optimal inoculum age and volume where xylitol concentration, yield and productivity were maximized. The optimal conditions of inoculation age and volume were 5.86 h and 11.66 % (v/v), respectively. Maximized results were observed at 48 h as compared to 72 h pre-optimization. Xylitol concentration slightly improved from 56 g/L to 59.71 g/L (<em>p</em>-value = 0.043). Yield improved from 0.56 g/g to 0.66 g/g (<em>p</em>-value = 0.044), whereas, productivity showed a significant increase from 0.76 g/L.h to 1.24 g/L.h (<em>p</em>-value = 0.008). Xylose Reductase activity improved by 1.67-folds and Xylitol Dehydrogenase activity decreased by 1.3 folds. This work suggests a simple inoculum strategy that could expedite the enzyme system required for xylitol production, enabling a 1.7-fold increase in productivity.</p></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":null,"pages":null},"PeriodicalIF":9.7000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Two-stage seeding strategy and its multi-response optimization for enhanced xylitol production by Debaryomyces nepalensis NCYC 3413\",\"authors\":\"\",\"doi\":\"10.1016/j.biortech.2024.131469\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The aim was to develop a two-stage seeding strategy and its optimization to enhance the conversion of xylose to xylitol by <em>Debaryomyces nepalensis</em> NCYC 3413. To develop efficient seed, multi-response optimization was employed to obtain optimal inoculum age and volume where xylitol concentration, yield and productivity were maximized. The optimal conditions of inoculation age and volume were 5.86 h and 11.66 % (v/v), respectively. Maximized results were observed at 48 h as compared to 72 h pre-optimization. Xylitol concentration slightly improved from 56 g/L to 59.71 g/L (<em>p</em>-value = 0.043). Yield improved from 0.56 g/g to 0.66 g/g (<em>p</em>-value = 0.044), whereas, productivity showed a significant increase from 0.76 g/L.h to 1.24 g/L.h (<em>p</em>-value = 0.008). Xylose Reductase activity improved by 1.67-folds and Xylitol Dehydrogenase activity decreased by 1.3 folds. This work suggests a simple inoculum strategy that could expedite the enzyme system required for xylitol production, enabling a 1.7-fold increase in productivity.</p></div>\",\"PeriodicalId\":258,\"journal\":{\"name\":\"Bioresource Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":9.7000,\"publicationDate\":\"2024-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioresource Technology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0960852424011738\",\"RegionNum\":1,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURAL ENGINEERING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0960852424011738","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}
Two-stage seeding strategy and its multi-response optimization for enhanced xylitol production by Debaryomyces nepalensis NCYC 3413
The aim was to develop a two-stage seeding strategy and its optimization to enhance the conversion of xylose to xylitol by Debaryomyces nepalensis NCYC 3413. To develop efficient seed, multi-response optimization was employed to obtain optimal inoculum age and volume where xylitol concentration, yield and productivity were maximized. The optimal conditions of inoculation age and volume were 5.86 h and 11.66 % (v/v), respectively. Maximized results were observed at 48 h as compared to 72 h pre-optimization. Xylitol concentration slightly improved from 56 g/L to 59.71 g/L (p-value = 0.043). Yield improved from 0.56 g/g to 0.66 g/g (p-value = 0.044), whereas, productivity showed a significant increase from 0.76 g/L.h to 1.24 g/L.h (p-value = 0.008). Xylose Reductase activity improved by 1.67-folds and Xylitol Dehydrogenase activity decreased by 1.3 folds. This work suggests a simple inoculum strategy that could expedite the enzyme system required for xylitol production, enabling a 1.7-fold increase in productivity.
期刊介绍:
Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies.
Topics include:
• Biofuels: liquid and gaseous biofuels production, modeling and economics
• Bioprocesses and bioproducts: biocatalysis and fermentations
• Biomass and feedstocks utilization: bioconversion of agro-industrial residues
• Environmental protection: biological waste treatment
• Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.