香蕉中的 RNA 沉默途径以内源性病毒元件为目标。

IF 8.3 1区 生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2024-09-18 DOI:10.1111/nph.20112
Pierre-Olivier Duroy, Jonathan Seguin, Sébastien Ravel, Rajeswaran Rajendran, Nathalie Laboureau, Frédéric Salmon, Jean-Marie Delos, Mikhail Pooggin, Marie-Line Iskra-Caruana, Matthieu Chabannes
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引用次数: 0

摘要

来自三个不同物种的内源香蕉条纹病毒(eBSV)整合子存在于芭蕉(Musa balbisiana)(B)而非尖嘴蕉(Musa acuminata)(A)的香蕉基因组中,它们能够在种间三倍体 AAB 香蕉杂交种中重组导致香蕉条纹病的功能性外显子病毒,但不能在二倍体(BB)母本系中重组,因为二倍体母本系含有相同的 eBSV 基因座。在这里,我们研究了这些 eBSV 的调控。使用 Illumina 和亚硫酸氢盐测序技术对无 eBSV 的 AAA 香蕉植株和带有 eBSV 的 BB 或 AAB 香蕉植株进行了深入分析,以确定来自 eBSV 的 siRNA、转录本和甲基化的特征。在 BB 植物中,eBSV 的 siRNA 积累序列和非积累序列都在 CG、CHG 和 CHH 三种上下文中被大量甲基化。我们的数据表明,在 BB 二倍体中,eBSV 在表观遗传水平上受到控制。这种调控不仅能防止它们的唤醒和对植物的系统性感染,还可能参与了 BB 植物对蚧壳虫传播的病毒感染的内在抵抗力。因此,这些发现对其他寄生综合病毒的植物资源也有意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Endogenous viral elements are targeted by RNA silencing pathways in banana

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来源期刊
New Phytologist
New Phytologist 生物-植物科学
自引率
5.30%
发文量
728
期刊介绍: New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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