{"title":"负载于脂质体纳米载体的黑升麻油对副丝状念珠菌分离物的抑制作用。","authors":"Ardalan Ghiaee Shamloo, Hossein Zarrinfar, Mahmoud Reza Jaafari, Mohammad Hossein Yadegari","doi":"10.18502/ijm.v16i4.16316","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong><i>Candida parapsilosis</i> is the second most common species causing infectious diseases and can lead to biofilm resistance. This study aims to adjust and synthesize a liposomal compound of <i>Nigella sativa</i> and evaluate its antifungal properties against <i>C. parapsilosis</i> isolates.</p><p><strong>Materials and methods: </strong>The liposomal formulation of <i>N. sativa</i> was optimized through the utilization of transmission electron microscopy (TEM), particle size analysis, zeta potential measurement, and UV-visible spectrophotometry. Furthermore, an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was conducted on peripheral blood mononuclear cells (PBMCs). The antifungal efficacy was evaluated in accordance with the M27-A3 guideline.</p><p><strong>Results: </strong>The minimum inhibitory concentrations (MICs) of <i>N. sativa</i> oil and the liposomal formulation on <i>C. parapsilosis</i> isolates ranged from 128 to 8 µg/mL and from 250 to 31.25 µg/mL, respectively. The MIC<sub>50</sub> and MIC<sub>90</sub> values of <i>N. sativa</i> oil and the liposomal formulation were 125, 187, and 32, 96 µg/mL, respectively. The viability percentage of cells treated with the liposomal formulation and free <i>N. sativa</i> oil was 91% and 85%, respectively.</p><p><strong>Conclusion: </strong>The cytotoxicity of free <i>N. sativa</i> was significantly reduced when using nanoliposomes. The liposomal form of <i>N. sativa</i> showed greater antifungal properties compared to the free <i>N. sativa</i> extract against <i>C. parapsilosis</i> isolates.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 4","pages":"560-568"},"PeriodicalIF":1.3000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11389775/pdf/","citationCount":"0","resultStr":"{\"title\":\"Inhibitory effect of <i>Nigella sativa</i> oil loaded to liposomal nanocarriers on <i>Candida parapsilosis</i> isolates.\",\"authors\":\"Ardalan Ghiaee Shamloo, Hossein Zarrinfar, Mahmoud Reza Jaafari, Mohammad Hossein Yadegari\",\"doi\":\"10.18502/ijm.v16i4.16316\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong><i>Candida parapsilosis</i> is the second most common species causing infectious diseases and can lead to biofilm resistance. This study aims to adjust and synthesize a liposomal compound of <i>Nigella sativa</i> and evaluate its antifungal properties against <i>C. parapsilosis</i> isolates.</p><p><strong>Materials and methods: </strong>The liposomal formulation of <i>N. sativa</i> was optimized through the utilization of transmission electron microscopy (TEM), particle size analysis, zeta potential measurement, and UV-visible spectrophotometry. Furthermore, an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was conducted on peripheral blood mononuclear cells (PBMCs). The antifungal efficacy was evaluated in accordance with the M27-A3 guideline.</p><p><strong>Results: </strong>The minimum inhibitory concentrations (MICs) of <i>N. sativa</i> oil and the liposomal formulation on <i>C. parapsilosis</i> isolates ranged from 128 to 8 µg/mL and from 250 to 31.25 µg/mL, respectively. The MIC<sub>50</sub> and MIC<sub>90</sub> values of <i>N. sativa</i> oil and the liposomal formulation were 125, 187, and 32, 96 µg/mL, respectively. The viability percentage of cells treated with the liposomal formulation and free <i>N. sativa</i> oil was 91% and 85%, respectively.</p><p><strong>Conclusion: </strong>The cytotoxicity of free <i>N. sativa</i> was significantly reduced when using nanoliposomes. The liposomal form of <i>N. sativa</i> showed greater antifungal properties compared to the free <i>N. sativa</i> extract against <i>C. parapsilosis</i> isolates.</p>\",\"PeriodicalId\":14633,\"journal\":{\"name\":\"Iranian Journal of Microbiology\",\"volume\":\"16 4\",\"pages\":\"560-568\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11389775/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijm.v16i4.16316\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v16i4.16316","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Inhibitory effect of Nigella sativa oil loaded to liposomal nanocarriers on Candida parapsilosis isolates.
Background and objectives: Candida parapsilosis is the second most common species causing infectious diseases and can lead to biofilm resistance. This study aims to adjust and synthesize a liposomal compound of Nigella sativa and evaluate its antifungal properties against C. parapsilosis isolates.
Materials and methods: The liposomal formulation of N. sativa was optimized through the utilization of transmission electron microscopy (TEM), particle size analysis, zeta potential measurement, and UV-visible spectrophotometry. Furthermore, an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was conducted on peripheral blood mononuclear cells (PBMCs). The antifungal efficacy was evaluated in accordance with the M27-A3 guideline.
Results: The minimum inhibitory concentrations (MICs) of N. sativa oil and the liposomal formulation on C. parapsilosis isolates ranged from 128 to 8 µg/mL and from 250 to 31.25 µg/mL, respectively. The MIC50 and MIC90 values of N. sativa oil and the liposomal formulation were 125, 187, and 32, 96 µg/mL, respectively. The viability percentage of cells treated with the liposomal formulation and free N. sativa oil was 91% and 85%, respectively.
Conclusion: The cytotoxicity of free N. sativa was significantly reduced when using nanoliposomes. The liposomal form of N. sativa showed greater antifungal properties compared to the free N. sativa extract against C. parapsilosis isolates.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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