负载于脂质体纳米载体的黑升麻油对副丝状念珠菌分离物的抑制作用。

IF 1.3 Q4 MICROBIOLOGY Iranian Journal of Microbiology Pub Date : 2024-08-01 DOI:10.18502/ijm.v16i4.16316
Ardalan Ghiaee Shamloo, Hossein Zarrinfar, Mahmoud Reza Jaafari, Mohammad Hossein Yadegari
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引用次数: 0

摘要

背景和目的:副丝状念珠菌是引起感染性疾病的第二大常见菌种,可导致生物膜抗药性。本研究旨在调整和合成一种黑升麻脂质体化合物,并评估其对副丝状念珠菌分离株的抗真菌特性:通过利用透射电子显微镜(TEM)、粒度分析、ZETA电位测量和紫外-可见分光光度法,优化了黑木耳脂质体配方。此外,还对外周血单核细胞(PBMCs)进行了 MTT(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)检测。抗真菌药效的评估符合 M27-A3 指南:结果:N. sativa 油和脂质体制剂对副丝状菌分离株的最小抑菌浓度(MICs)分别为 128 至 8 µg/mL 和 250 至 31.25 µg/mL。荠菜油和脂质体制剂的 MIC50 和 MIC90 值分别为 125、187 和 32、96 µg/mL。用脂质体制剂和游离荠菜油处理的细胞存活率分别为 91% 和 85%:结论:使用纳米脂质体可显著降低游离萝芙木油的细胞毒性。与游离 N. sativa 提取物相比,脂质体形式的 N. sativa 对 C. parapsilosis 分离物具有更强的抗真菌特性。
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Inhibitory effect of Nigella sativa oil loaded to liposomal nanocarriers on Candida parapsilosis isolates.

Background and objectives: Candida parapsilosis is the second most common species causing infectious diseases and can lead to biofilm resistance. This study aims to adjust and synthesize a liposomal compound of Nigella sativa and evaluate its antifungal properties against C. parapsilosis isolates.

Materials and methods: The liposomal formulation of N. sativa was optimized through the utilization of transmission electron microscopy (TEM), particle size analysis, zeta potential measurement, and UV-visible spectrophotometry. Furthermore, an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was conducted on peripheral blood mononuclear cells (PBMCs). The antifungal efficacy was evaluated in accordance with the M27-A3 guideline.

Results: The minimum inhibitory concentrations (MICs) of N. sativa oil and the liposomal formulation on C. parapsilosis isolates ranged from 128 to 8 µg/mL and from 250 to 31.25 µg/mL, respectively. The MIC50 and MIC90 values of N. sativa oil and the liposomal formulation were 125, 187, and 32, 96 µg/mL, respectively. The viability percentage of cells treated with the liposomal formulation and free N. sativa oil was 91% and 85%, respectively.

Conclusion: The cytotoxicity of free N. sativa was significantly reduced when using nanoliposomes. The liposomal form of N. sativa showed greater antifungal properties compared to the free N. sativa extract against C. parapsilosis isolates.

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来源期刊
CiteScore
2.40
自引率
7.10%
发文量
96
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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