Beata Czarczynska-Goslinska, Tomasz Goslinski, Agata Roszak, Anna Froelich, Piotr Szyk, Dariusz T Mlynarczyk, Lukasz Sobotta, Irena Budnik, Oliwia Kordyl, Tomasz Osmałek
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引用次数: 0
摘要
文章旨在概述用微针治疗恶性皮肤癌的潜力,微针上覆盖着含有光敏剂-原卟啉 IX 二钠盐(PPIX)的聚合物层。立体光刻(SLA)是三维打印技术的一种,它在制备含有原卟啉 IX 的微针系统方面的实用性已得到证实。利用 SLA 方法可以打印出金字塔形的微针,微针表面覆盖有三种不同的 0.1% PPIX 水凝胶,分别基于海藻酸钠、黄原胶和聚氧乙烯醚。对这些水凝胶进行了流变学测试和显微分析。涂有两层含有 0.1% PPIX 的聚氧乙烯水凝胶的微针在弗兰兹扩散细胞中进行了释放测试。PPIX 的释放量最初有所增加,然后保持相对稳定。在三个弗兰兹细胞中进行四小时测试后,释放的物质为 0.2569 ± 0.0683 mg/cm2。此外,还使用 Microtox 系统评估了这种微针的急性毒性。结果表明,进一步开发研究作为光敏剂载体的微针是有用的。
Microneedle System Coated with Hydrogels Containing Protoporphyrin IX for Potential Application in Pharmaceutical Technology.
The article aims to outline the potential of treating malignant skin cancer with microneedles covered with polymer layers containing a photosensitizer-protoporphyrin IX disodium salt (PPIX). The usefulness of stereolithography (SLA), which is a form of 3D-printing technology, for the preparation of a microneedle system with protoporphyrin IX was demonstrated. The SLA method allowed for pyramid-shaped microneedles to be printed that were covered with three different 0.1% PPIX hydrogels based on sodium alginate, xanthan, and poloxamer. Rheological tests and microscopic analysis of the hydrogels were performed. Microneedles coated with two layers of poloxamer-based hydrogel containing 0.1% PPIX were subjected to release tests in Franz diffusion cells. The release profile of PPIX initially increased and then remained relatively constant. The amount of substance released after a four-hour test in three Franz cells was 0.2569 ± 0.0683 mg/cm2. Moreover, the acute toxicity of this type of microneedle was assessed using the Microtox system. The obtained results show the usefulness of further development studies on microneedles as carriers of photosensitizing agents.