慢性乙型肝炎病毒感染者的 MTHFR 基因多态性、MTRR 基因多态性和 HBV 基因 BCP 1762/1764 突变与疾病进展的相互关系。

IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-09-19 DOI:10.1080/15257770.2024.2406223
Qiu Shunhua, Jin Lifen, Yang Dan, Zhang Dewen
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引用次数: 0

摘要

目的:慢性乙型肝炎病毒(HBV)感染是严重影响患者健康的主要疾病:慢性乙型肝炎病毒(HBV)感染是严重影响患者健康的主要疾病。本文研究了 MTHFR 基因多态性、MTRR 基因多态性和 HBV 基因 BCP 区 1762/1764 突变与慢性 HBV 患者疾病进展的关系:将自贡市第三人民医院2021年1月至2022年6月的144例慢性HBV感染患者作为研究对象。这些患者被分为乙肝原发性肝癌患者组(PLC)51例,非原发性肝癌患者组(Non-PLC)93例,非PLC又分为慢性乙肝病毒携带者(CHC)49例,乙肝活肝硬化(LC)44例。通过 PCR 溶解曲线检测 MTHFR (C677T)、MTRR (A66G) 和 MTHFR (A1298C) 基因的多态性。通过实时 PCR 对 HBV-DNA 水平进行定量,并通过 ARMS-PCR 检测 HBV 基因 BCP 区的 1762/1764 突变位点。数据采用 SPSS 统计软件进行统计分析:PLC组BCP区1762/1764位点的HBV基因突变比例为82.4%,高于LC组(63.6%)和CHC组(51.0%),差异有统计学意义(P>0.05)。不同阶段(CHC、LC 和 PLC)慢性乙型肝炎病毒感染患者的 MTHFR C677T、MTRR A66G 和 MTHFR A1298C 基因多态性分布显示,组间和组内无性别和年龄差异(P > 0.05)。在 MTHFR 677CT + TT、MTRR 66AG + GG 和 MTHFR 1298AA 基因型患者中,PLC 组 BCP 区 1762/1764 的 HBV 突变比例高于 CHC 组和 LC 组,差异有统计学意义(P P P 结论):慢性乙型肝炎病毒感染者的 MTHFR C677T、MTRR A66G 和 MTHFR A1298C 基因多态性分布无性别和年龄差异。HBV 基因 BCP 区 1762/1764 突变可能与慢性 HBV 感染者肝癌的发生和发展有关。MTHFR C677T、MTHFR A1298C 和 MTRR A66G 基因多态性的单一差异可能对慢性 HBV 感染者的疾病进展影响不大。MTHFR 677CT + TT、MTRR 66AG + GG 和 MTHFR 1298AA 基因型与 HBV 基因 BCP 区 1762/1764 突变可能与乙型肝炎肝癌的发生和发展密切相关。
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Interrelationships among MTHFR gene polymorphisms, MTRR gene polymorphisms, and HBV gene BCP 1762/1764 mutations with disease progression in Chronic hepatitis B virus infection patients.

Objective: Chronic hepatitis B virus (HBV) infection is a major disease that seriously affects the health of patients. In this paper, the relationship among MTHFR gene polymorphism, MTRR gene polymorphism and 1762/1764 mutation in the BCP region of HBV gene with disease progression in chronic HBV patients was studied.

Methods: A total of 144 chronic HBV infection patients from January 2021 to June 2022 in the Third People's Hospital of Zigong City, were included as the study subjects. These patients were divided into hepatitis B primary liver cancer patients group (PLC) in 51 cases, Non-primary liver cancer patients group (Non-PLC) in 93 cases, Non-PLC is also divided into chronic hepatitis B virus carriers (CHC) in 49 cases, hepatitis B Live cirrhosis(LC) in 44 cases. MTHFR (C677T), MTRR (A66G) and MTHFR (A1298C) genes polymorphisms were detected by PCR-dissolution curve. The level of HBV-DNA was quantified by real-time PCR, and the 1762/1764 mutation site in the BCP region of the HBV gene were detected by ARMS-PCR. Data were statistically analyzed using the SPSS statistical software.

Results: The proportion of HBV mutations in BCP region 1762/1764 in PLC group was 82.4%, which was higher than that in LC group (63.6%) and CHC group (51.0%), and the differences were statistically significant (p < 0.05). There were no significant differences in the distribution of MTHFR C677T, MTHFR A1298C and MTRR A66G polymorphisms among CHC, LC and PLC (p > 0.05). The polymorphism distribution of MTHFR C677T, MTRR A66G and MTHFR A1298C genes in patients with chronic hepatitis B virus infection at different stages (CHC, LC and PLC) showed no gender or age differences between and within groups (p > 0.05). Among the patients with MTHFR 677CT + TT, MTRR 66AG + GG and MTHFR 1298AA genotype, the proportion of HBV mutation in BCP region 1762/1764 in PLC group was higher than that in CHC group and LC group, and the differences were statistically significant (p < 0.05). Folate levels in the PLC group were lower than those in the non-PLC group (CHC and LC patients), and the difference was statistically significant compared with the CHC group (p < 0.05). In different MTHFR C677T and MTRR A66G genotypes, the serum GGT activity were statistically significant between mutant PLC and mutant Non-PLC (p < 0.05).

Conclusion: MTHFR C677T, MTRR A66G and MTHFR A1298C gene polymorphisms distribution have no gender and age differences in chronic hepatitis B virus infection patients. The mutation of HBV gene BCP region 1762/1764 may be associated with the occurrence and development of liver cancer in patients with chronic HBV infection. Single difference of MTHFR C677T, MTHFR A1298C and MTRR A66G gene polymorphisms may have little effect on the disease progression in patients with chronic HBV infection. MTHFR 677CT + TT, MTRR 66AG + GG and MTHFR 1298AA genotype combined with HBV gene BCP region 1762/1764 mutation may be closely related to the occurrence and development of hepatitis B liver cancer.

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来源期刊
Nucleosides, Nucleotides & Nucleic Acids
Nucleosides, Nucleotides & Nucleic Acids 生物-生化与分子生物学
CiteScore
2.60
自引率
7.70%
发文量
91
审稿时长
6 months
期刊介绍: Nucleosides, Nucleotides & Nucleic Acids publishes research articles, short notices, and concise, critical reviews of related topics that focus on the chemistry and biology of nucleosides, nucleotides, and nucleic acids. Complete with experimental details, this all-inclusive journal emphasizes the synthesis, biological activities, new and improved synthetic methods, and significant observations related to new compounds.
期刊最新文献
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