越南湄公河三角洲牛群中块状皮肤病病毒的特征和分子鉴定。

IF 0.9 Q3 VETERINARY SCIENCES Open Veterinary Journal Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI:10.5455/OVJ.2024.v14.i8.16
Tran Ngoc Bich, Le Quang Trung, Truong Van Hieu, Vo Tuan Khai Huyen, Thai Quoc Hieu, Huynh Truong Giang, Nguyen Tran Phuoc Chien
{"title":"越南湄公河三角洲牛群中块状皮肤病病毒的特征和分子鉴定。","authors":"Tran Ngoc Bich, Le Quang Trung, Truong Van Hieu, Vo Tuan Khai Huyen, Thai Quoc Hieu, Huynh Truong Giang, Nguyen Tran Phuoc Chien","doi":"10.5455/OVJ.2024.v14.i8.16","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Lumpy skin disease (LSD) is caused by a virus belonging to the genus <i>Capripoxvirus</i>, exhibiting clinical symptoms ranging from mild signs to the development of nodules. LSD emerged in Asia and Southeast Asia, including Vietnam, in October 2020 and has since spread throughout the region, resulting in productivity and economic losses.</p><p><strong>Aim: </strong>This study aimed to investigate the virus-causing papular dermatitis in cattle from the Mekong Delta region of Vietnam by analyzing its GPCR gene and assessing its evolutionary relationship with sequences in the GenBank database.</p><p><strong>Methods: </strong>Blood samples (<i>n</i> = 180) were collected from cattle farms in Ben Tre, Tien Giang, and Tra Vinh provinces. PCR targeting the P32 antigen gene was utilized to detect LSDV presence, and GPCR gene amplification was performed to assess genetic variability.</p><p><strong>Results: </strong>LSDV was detected in 8.33% (15/180) of the samples using PCR targeting the P32 antigen gene. Each sample that tested positive for LSDV demonstrated complete amplification of the GPCR gene. Sequence alignments and phylogenetic analyses of the GPCR gene revealed that Mekong Delta LSDV isolates shared genetic similarities and possessed a 12-nucleotide insertion comparable to strains from China in 2019 and Northern Vietnam in 2020.</p><p><strong>Conclusion: </strong>This study provides preliminary insights into the molecular characteristics of LSDV in cattle from the Mekong Delta region of Vietnam. The observed genetic relatedness to other LSDV sequences from Asia and Southeast Asia underscores the importance of regional surveillance and control measures. These findings contribute to the development of effective strategies for LSDV control and prevention.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415901/pdf/","citationCount":"0","resultStr":"{\"title\":\"Characterization and molecular identification of the lumpy skin disease virus in cattle in the Mekong Delta of Vietnam.\",\"authors\":\"Tran Ngoc Bich, Le Quang Trung, Truong Van Hieu, Vo Tuan Khai Huyen, Thai Quoc Hieu, Huynh Truong Giang, Nguyen Tran Phuoc Chien\",\"doi\":\"10.5455/OVJ.2024.v14.i8.16\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Lumpy skin disease (LSD) is caused by a virus belonging to the genus <i>Capripoxvirus</i>, exhibiting clinical symptoms ranging from mild signs to the development of nodules. LSD emerged in Asia and Southeast Asia, including Vietnam, in October 2020 and has since spread throughout the region, resulting in productivity and economic losses.</p><p><strong>Aim: </strong>This study aimed to investigate the virus-causing papular dermatitis in cattle from the Mekong Delta region of Vietnam by analyzing its GPCR gene and assessing its evolutionary relationship with sequences in the GenBank database.</p><p><strong>Methods: </strong>Blood samples (<i>n</i> = 180) were collected from cattle farms in Ben Tre, Tien Giang, and Tra Vinh provinces. PCR targeting the P32 antigen gene was utilized to detect LSDV presence, and GPCR gene amplification was performed to assess genetic variability.</p><p><strong>Results: </strong>LSDV was detected in 8.33% (15/180) of the samples using PCR targeting the P32 antigen gene. Each sample that tested positive for LSDV demonstrated complete amplification of the GPCR gene. Sequence alignments and phylogenetic analyses of the GPCR gene revealed that Mekong Delta LSDV isolates shared genetic similarities and possessed a 12-nucleotide insertion comparable to strains from China in 2019 and Northern Vietnam in 2020.</p><p><strong>Conclusion: </strong>This study provides preliminary insights into the molecular characteristics of LSDV in cattle from the Mekong Delta region of Vietnam. The observed genetic relatedness to other LSDV sequences from Asia and Southeast Asia underscores the importance of regional surveillance and control measures. These findings contribute to the development of effective strategies for LSDV control and prevention.</p>\",\"PeriodicalId\":19531,\"journal\":{\"name\":\"Open Veterinary Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415901/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Open Veterinary Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/OVJ.2024.v14.i8.16\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/31 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/OVJ.2024.v14.i8.16","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/31 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

背景:结节性皮肤病(LSD)是由一种属于Capripoxvirus属的病毒引起的,临床症状从轻微症状到出现结节不等。LSD于2020年10月在包括越南在内的亚洲和东南亚地区出现,此后在整个地区蔓延,造成了生产力和经济损失。目的:本研究旨在通过分析其GPCR基因并评估其与GenBank数据库中序列的进化关系,调查导致越南湄公河三角洲地区牛患丘疹性皮炎的病毒:方法:从槟椥省、前江省和茶荣省的养牛场采集血液样本(n = 180)。利用针对 P32 抗原基因的 PCR 检测 LSDV 的存在,并进行 GPCR 基因扩增以评估遗传变异:结果:利用针对 P32 抗原基因的 PCR,在 8.33% 的样本(15/180)中检测到 LSDV。对 LSDV 检测呈阳性的每个样本都显示出 GPCR 基因的完全扩增。GPCR 基因的序列比对和系统进化分析表明,湄公河三角洲 LSDV 分离物与 2019 年来自中国和 2020 年来自越南北部的菌株具有遗传相似性,并具有 12 个核苷酸插入:本研究初步揭示了越南湄公河三角洲地区牛群中 LSDV 的分子特征。观察到的与亚洲和东南亚其他 LSDV 序列的遗传相关性强调了区域监测和控制措施的重要性。这些发现有助于制定有效的 LSDV 防控策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Characterization and molecular identification of the lumpy skin disease virus in cattle in the Mekong Delta of Vietnam.

Background: Lumpy skin disease (LSD) is caused by a virus belonging to the genus Capripoxvirus, exhibiting clinical symptoms ranging from mild signs to the development of nodules. LSD emerged in Asia and Southeast Asia, including Vietnam, in October 2020 and has since spread throughout the region, resulting in productivity and economic losses.

Aim: This study aimed to investigate the virus-causing papular dermatitis in cattle from the Mekong Delta region of Vietnam by analyzing its GPCR gene and assessing its evolutionary relationship with sequences in the GenBank database.

Methods: Blood samples (n = 180) were collected from cattle farms in Ben Tre, Tien Giang, and Tra Vinh provinces. PCR targeting the P32 antigen gene was utilized to detect LSDV presence, and GPCR gene amplification was performed to assess genetic variability.

Results: LSDV was detected in 8.33% (15/180) of the samples using PCR targeting the P32 antigen gene. Each sample that tested positive for LSDV demonstrated complete amplification of the GPCR gene. Sequence alignments and phylogenetic analyses of the GPCR gene revealed that Mekong Delta LSDV isolates shared genetic similarities and possessed a 12-nucleotide insertion comparable to strains from China in 2019 and Northern Vietnam in 2020.

Conclusion: This study provides preliminary insights into the molecular characteristics of LSDV in cattle from the Mekong Delta region of Vietnam. The observed genetic relatedness to other LSDV sequences from Asia and Southeast Asia underscores the importance of regional surveillance and control measures. These findings contribute to the development of effective strategies for LSDV control and prevention.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Open Veterinary Journal
Open Veterinary Journal VETERINARY SCIENCES-
CiteScore
1.40
自引率
0.00%
发文量
112
审稿时长
12 weeks
期刊介绍: Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
期刊最新文献
In vivo assessment of the anti-benign prostatic hyperplasia effect of hot ethanolic extract of flax seeds in male rats. A rare case of "Hawkins type 3" talar neck fracture in a cat. Ameliorative effect of transfersome gel of kepok banana peel extract (Musa balbisiana) against photoaging in Wistar rat skin. Antimalarial activity of borrelidin and fumagilin in Plasmodium berghei-infected mice. Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1