人体血清中 PDE5 浓度的测量:对照组和前列腺癌患者的概念验证和方法验证。

IF 5.4 2区 医学 Q1 Medicine Journal of Endocrinological Investigation Pub Date : 2024-10-01 DOI:10.1007/s40618-024-02428-w
Giovanni Luca Gravina, Eugenia Guida, Maria Dri, Renato Massoud, Savino M Di Stasi, Giorgio Fucci, Andrea Sansone, Susanna Dolci, Emmanuele A Jannini
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引用次数: 0

摘要

目的:我们的目的是研究能否在人体血清中检测和定量 5 型磷酸二酯酶(PDE5):方法:采集对照组男女受试者的血样。方法:采集对照组男性和女性受试者的血样,用特定的酶联免疫吸附试剂盒检测 PDE5 的水平。采用年龄和血清中 PSA(男性受试者)或年龄(女性受试者)水平加权的 ROC 曲线来确定检测 PCa 的预测能力。根据 ROC 曲线分析中确定的临界值,确定敏感性、特异性、PPV 和 NPV 值:研究对象包括41名对照组男性受试者、18名对照组女性受试者和55名连续受试者,其中25人患有良性前列腺肥大(BPH),30人经组织学证实患有前列腺癌(PCa)。所有受试者的血清中均可检测到 PDE5 水平(范围:5 至 65 纳克/毫升)。通过 MANCOVA 分析发现,对照组或增生患者与 PCa 患者的血清 PDE5 存在显著差异。血清 PDE5 浓度的边际均值显示出显著差异(p 结论:PDE5 浓度的边际均值与 PCa 患者血清 PDE5 浓度的边际均值存在显著差异:我们首次证明,人体血清中可检测到 PDE5 水平,而且 PCa 患者的 PDE5 浓度明显高于良性前列腺增生患者或男女对照组。虽然血清中 PDE5 水平的测量可能会开辟新的研究途径,但 PCa 患者体内 PDE5 水平的临床意义值得进一步研究。
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Measurement of PDE5 concentration in human serum: proof-of-concept and validation of methodology in control and prostate cancer patients.

Purpose: We aimed to investigate if the type 5 phosphodiesterase (PDE5), an enzyme with cardinal biological functions in sexual and cardiovascular health, can be detected and quantited in human serum.

Methods: Blood samples were collected from control male and female subjects. PDE5 levels were measured by a specific ELISA kit. ROC curves weighted for age and serum levels of PSA (male subjects), or age (female subjects) were used to identify the predictive ability in the detection of PCa. Sensitivity, specificity, PPV and NPV values were determined for cut-off value determined during ROC curve analysis.

Results: 41 control male subjects, 18 control female subjects, and 55 consecutive subjects, of which 25 were affected by benign prostatic hypertrophy (BPH) and 30 with histologically confirmed prostate cancer (PCa), were studied. PDE5 serum levels were detectable in all subjects (range: 5 to 65 ng/ml). Analysis by MANCOVA identified a significant difference in serum PDE5 between control subjects or hyperplasia patients and PCa patients. Marginal means of serum PDE5 concentrations showed a significant difference (p < 0.001). The ROC curve demonstrated that PDE5 serum levels can predict men with or without PCa, with 0.806 AUC value (p < 0.0001). Using a 12.705 ng/ml PDE5 serum cut-off yielded sensitivity, specificity, PPV, and NPV of 83.3%, 77.27%, 62.5%, and 91.1% in detecting men with histologically proven PCa, respectively.

Conclusions: We demonstrated, for the first time, that PDE5 levels can be detected in human sera and that PCa patients have significantly higher PDE5 concentration compared to BPH patients or male and female controls. While serum PDE5 level measurement may open new research avenues, the clinical relevance of PDE5 levels in PCa patients deserves further investigation.

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来源期刊
Journal of Endocrinological Investigation
Journal of Endocrinological Investigation ENDOCRINOLOGY & METABOLISM-
CiteScore
8.10
自引率
7.40%
发文量
242
期刊介绍: The Journal of Endocrinological Investigation is a well-established, e-only endocrine journal founded 36 years ago in 1978. It is the official journal of the Italian Society of Endocrinology (SIE), established in 1964. Other Italian societies in the endocrinology and metabolism field are affiliated to the journal: Italian Society of Andrology and Sexual Medicine, Italian Society of Obesity, Italian Society of Pediatric Endocrinology and Diabetology, Clinical Endocrinologists’ Association, Thyroid Association, Endocrine Surgical Units Association, Italian Society of Pharmacology.
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