从堇菜中鉴定异喹啉生物碱及其乙酰胆碱酯酶抑制作用。

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL Fitoterapia Pub Date : 2024-09-24 DOI:10.1016/j.fitote.2024.106220

Meng Ren , Zixuan Wang , Jie Song , Yurun Wang , Taoshuai Cao , Xiangdong Qin , Du-Qiang Luo , Jun Zhang

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引用次数: 0

摘要

从 Corydalis mucronifera Maxim 中分离出了四种新的螺苄基异喹啉短叶木犀草素 N - Q（1-4）和一种罕见的氯化异喹啉短叶木犀草素 R（5）。根据 HRESIMS、1D 和 2D NMR 的大量光谱数据分析，阐明了它们的结构，并通过 ECD 数据确认了它们的绝对构型。对分离出的化合物进行了乙酰胆碱酯酶（AChE）抑制活性评估。与加兰他敏（1.34 μM）相比，Mucroniferanine R 显示出显著的活性，其 IC50 值为 0.78 μM。分子对接分析表明，木犀草素 R 与人 AChE 的活性位点相容，从而进一步证实了其 AChE 抑制活性。

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Identification of isoquinoline alkaloids from Corydalis mucronifera and their acetylcholinesterase inhibitory effects

Four new spirobenzylisoquinoline mucroniferanines N − Q (1–4) and a rare chlorinated isoquinoline mucroniferanine R (5) were isolated from Corydalis mucronifera Maxim. Their structures were elucidated based on extensive spectroscopic data analysis of HRESIMS, 1D and 2D NMR, and their absolute configurations were confirmed by ECD data. The isolated compounds were evaluated for acetylcholinesterase (AChE) inhibitory activities. Mucroniferanine R showed significant activities with IC₅₀ values of 0.78 μM compared to galanthamine (1.34 μM). The AChE inhibitory activity was further supported by the molecular docking analysis that exhibited the accommodation of mucroniferanine R in the active site of human AChE.

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.