{"title":"Oryza sativa 和 Oryza meridionalis 杂交后代中两个重复基因的组合导致种子流产。","authors":"Daiki Toyomoto, Yukika Shibata, Masato Uemura, Satoru Taura, Tadashi Sato, Robert Henry, Ryuji Ishikawa, Katsuyuki Ichitani","doi":"10.1270/jsbbs.23084","DOIUrl":null,"url":null,"abstract":"<p><p>Seed development is an essential phenomenon for all sexual propagative plant species. The functional allele at <i>SEED DEVELOPMENT 1</i> (<i>SDV1</i>) or <i>SEED DEVELOPMENT 2</i> (<i>SDV2</i>) loci is essential for seed development for <i>Oryza sativa</i> and <i>Oryza meridionalis</i>. In the present study, we performed fine mapping of <i>SDV1</i>, narrowing down the area of interest to 333kb on chromosome 6. Haplotype analysis around the <i>SDV1</i> locus of <i>O. meridionalis</i> accessions indicated that they shared the DNA polymorphism, suggesting that they have a common abortive allele at the <i>SDV1</i> locus. Linkage analysis of the candidate <i>SDV2</i> gene showed that it was located on chromosome 4. The candidate <i>SDV2</i> was confirmed using a population in which both the <i>SDV1</i> and <i>SDV2</i> genes were segregating. The chromosomal region covering the <i>SDV1</i> gene was predicted to contain 30 protein-coding genes in <i>O. sativa</i>. Five of these genes have conserved DNA sequences in the chromosomal region of the <i>SDV2</i> gene on chromosome 4, and not on chromosome 6, of <i>O. meridionalis</i>. These results suggest that these five genes could be candidates for <i>SDV1</i>, and that their orthologous genes located on chromosome 4 of <i>O. meridionalis</i> could be candidates for <i>SDV2</i>.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"74 2","pages":"146-158"},"PeriodicalIF":2.0000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11442109/pdf/","citationCount":"0","resultStr":"{\"title\":\"Seed abortion caused by the combination of two duplicate genes in the progeny from the cross between <i>Oryza sativa</i> and <i>Oryza meridionalis</i>.\",\"authors\":\"Daiki Toyomoto, Yukika Shibata, Masato Uemura, Satoru Taura, Tadashi Sato, Robert Henry, Ryuji Ishikawa, Katsuyuki Ichitani\",\"doi\":\"10.1270/jsbbs.23084\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Seed development is an essential phenomenon for all sexual propagative plant species. The functional allele at <i>SEED DEVELOPMENT 1</i> (<i>SDV1</i>) or <i>SEED DEVELOPMENT 2</i> (<i>SDV2</i>) loci is essential for seed development for <i>Oryza sativa</i> and <i>Oryza meridionalis</i>. In the present study, we performed fine mapping of <i>SDV1</i>, narrowing down the area of interest to 333kb on chromosome 6. Haplotype analysis around the <i>SDV1</i> locus of <i>O. meridionalis</i> accessions indicated that they shared the DNA polymorphism, suggesting that they have a common abortive allele at the <i>SDV1</i> locus. Linkage analysis of the candidate <i>SDV2</i> gene showed that it was located on chromosome 4. The candidate <i>SDV2</i> was confirmed using a population in which both the <i>SDV1</i> and <i>SDV2</i> genes were segregating. The chromosomal region covering the <i>SDV1</i> gene was predicted to contain 30 protein-coding genes in <i>O. sativa</i>. Five of these genes have conserved DNA sequences in the chromosomal region of the <i>SDV2</i> gene on chromosome 4, and not on chromosome 6, of <i>O. meridionalis</i>. These results suggest that these five genes could be candidates for <i>SDV1</i>, and that their orthologous genes located on chromosome 4 of <i>O. meridionalis</i> could be candidates for <i>SDV2</i>.</p>\",\"PeriodicalId\":9258,\"journal\":{\"name\":\"Breeding Science\",\"volume\":\"74 2\",\"pages\":\"146-158\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11442109/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Breeding Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1270/jsbbs.23084\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/4/2 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Breeding Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1270/jsbbs.23084","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/4/2 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
摘要
种子发育是所有有性繁殖植物物种的基本现象。种子发育 1（SDV1）或种子发育 2（SDV2）基因座上的功能等位基因对禾本科植物和经果类植物的种子发育至关重要。在本研究中，我们对 SDV1 进行了精细作图，将感兴趣的区域缩小到 6 号染色体上的 333kb。围绕 SDV1 基因座的单倍型分析表明，O. meridionalis 加入的基因具有共同的 DNA 多态性，这表明它们在 SDV1 基因座上有一个共同的失效等位基因。对候选 SDV2 基因的连锁分析表明，该基因位于第 4 号染色体上。利用 SDV1 和 SDV2 基因均发生分离的群体对候选 SDV2 基因进行了确认。据预测，覆盖 SDV1 基因的染色体区域在 O. sativa 中包含 30 个蛋白质编码基因。其中有五个基因的DNA序列与O. meridionalis的SDV2基因的染色体区域一致，SDV2基因位于4号染色体上，而不在6号染色体上。这些结果表明，这五个基因可能是 SDV1 的候选基因，而它们位于 O. meridionalis 第 4 号染色体上的同源基因可能是 SDV2 的候选基因。
Seed abortion caused by the combination of two duplicate genes in the progeny from the cross between Oryza sativa and Oryza meridionalis.
Seed development is an essential phenomenon for all sexual propagative plant species. The functional allele at SEED DEVELOPMENT 1 (SDV1) or SEED DEVELOPMENT 2 (SDV2) loci is essential for seed development for Oryza sativa and Oryza meridionalis. In the present study, we performed fine mapping of SDV1, narrowing down the area of interest to 333kb on chromosome 6. Haplotype analysis around the SDV1 locus of O. meridionalis accessions indicated that they shared the DNA polymorphism, suggesting that they have a common abortive allele at the SDV1 locus. Linkage analysis of the candidate SDV2 gene showed that it was located on chromosome 4. The candidate SDV2 was confirmed using a population in which both the SDV1 and SDV2 genes were segregating. The chromosomal region covering the SDV1 gene was predicted to contain 30 protein-coding genes in O. sativa. Five of these genes have conserved DNA sequences in the chromosomal region of the SDV2 gene on chromosome 4, and not on chromosome 6, of O. meridionalis. These results suggest that these five genes could be candidates for SDV1, and that their orthologous genes located on chromosome 4 of O. meridionalis could be candidates for SDV2.
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