全面分析骨肉瘤中细胞周期蛋白依赖性蛋白激酶家族的表达和预后。

IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2024-10-02 DOI:10.1080/15257770.2024.2410957
Jianshui Mao, Hui-Min Li, Zhidan Huang
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引用次数: 0

摘要

背景和目的:细胞周期蛋白依赖性蛋白激酶(CDK)被认为是未来的治疗靶点,因为它们控制着对癌细胞的存活和生长至关重要的过程。然而,目前仍缺乏对骨肉瘤中 CDK 不同表达谱和预后因素的研究:方法:从基因表达总库(GEO)数据库(GSE42352)中检索了骨肉瘤微RNA(GSE65071)和基因表达谱。利用 TARGET 数据库发现了 CDK 在预后方面的巨大差异。利用Cytoscape构建了miRNAs-CDKs网络,并完成了功能和通路富集分析。结果显示,在骨肉瘤患者中,miRNAs与CDKs之间存在相互作用:结果:与正常样本相比,骨肉瘤患者的CDK1-5、CDK18、CDK16和CDK17基因表达水平明显较高,而CDK7-9、CDK11B、CDK16和CDK20基因表达水平则明显较低。CDK3和18基因水平较低或CDK6、9基因水平较高的骨肉瘤患者预后良好,预期寿命较长。免疫检查点基因、m6A相关基因的表达和CDKs的表达都有一定的联系。最后,我们构建了一个重要的CDKs和miRNAs网络:根据我们的研究,CDK3、6、9和18已被确定为骨肉瘤的可能治疗靶点,CDKs可能在控制肿瘤细胞中的m6A突变以及免疫检查点调控中发挥作用。
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Comprehensive analysis of the expression and prognosis for cyclin-dependent protein kinase family in osteosarcoma.

Background and objective: Cyclin-dependent protein kinases (CDKs) have been suggested as prospective therapeutic targets because they control processes vital to the survival and growth of cancer cells. However, research on the varied CDK expression profiles and prognostic factors in osteosarcoma is still lacking.

Methods: The osteosarcoma microRNA (GSE65071) and gene expression profiles were retrieved from the Gene Expression Omnibus (GEO) database (GSE42352). A substantial variation in prognosis was discovered in CDKs using the TARGET database. Cytoscape was used to construct the miRNAs-CDKs network, and functional and pathway enrichment analyses were completed. It was looked at how immune checkpoint genes, m6A-related genes, and CDKs interact.

Results: In patients with osteosarcoma compared to normal samples, CDK1-5, CDK18, CDK16, and CDK17 gene expression levels were considerably greater, whereas CDK7-9, CDK11B, CDK16, and CDK20 gene expression levels were significantly lower. Patients with osteosarcoma who had low CDK3 and 18 gene levels or high CDK6, 9 gene levels were predicted to have a favorable prognosis and a long-life expectancy. Immune checkpoint genes, m6A-related gene expression, and CDKs expression all showed some connection. Finally, a network of crucial CDKs and miRNAs was constructed.

Conclusion: According to our research, CDK3, 6, 9, and 18 have been identified as possible therapeutic targets for osteosarcoma, and CDKs may have a role in controlling m6A mutations in tumor cells as well as immune checkpoint regulation.

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来源期刊
Nucleosides, Nucleotides & Nucleic Acids
Nucleosides, Nucleotides & Nucleic Acids 生物-生化与分子生物学
CiteScore
2.60
自引率
7.70%
发文量
91
审稿时长
6 months
期刊介绍: Nucleosides, Nucleotides & Nucleic Acids publishes research articles, short notices, and concise, critical reviews of related topics that focus on the chemistry and biology of nucleosides, nucleotides, and nucleic acids. Complete with experimental details, this all-inclusive journal emphasizes the synthesis, biological activities, new and improved synthetic methods, and significant observations related to new compounds.
期刊最新文献
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