开发一种用于全面分析尼古丁和烟草制品使用者暴露组的 LC-HRMS 非靶向方法--展示吸烟者的情况。

IF 2.8 3区 医学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Chromatography B Pub Date : 2024-09-29 DOI:10.1016/j.jchromb.2024.124330
Alpeshkumar Kachhadia, Therese Burkhardt, Gerhard Scherer, Max Scherer, Nikola Pluym
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引用次数: 0

摘要

在过去十年中,电子香烟、加热烟草制品和其他无烟替代品在全球的普及率大幅增长。这些产品被建议作为香烟等传统烟草产品的免燃烧替代品,旨在减少与吸烟相关的负面健康影响。然而,这些产品对普通人群健康和安全的影响仍不明确,因为这些产品的绝对暴露量尚未得到深入研究。本项目开发了一种非靶向 LC-HRMS 方法,包括四种不同的分析模式,用于调查产品使用者尿液中的暴露概况。该方法的特点是灵敏度高、重现性好,这一点在方法验证过程中已得到证实。作为概念验证,我们首先应用该方法检测了吸烟者和非吸烟者之间生物标志物暴露(BoEs)的显著差异。我们共观察到 171 种生物标志物在吸烟者中明显升高,其中包括几种众所周知的烟雾暴露生物标志物,如尼古丁及其代谢物、巯基酸衍生物和酚类化合物。其中一些检测到的生物标志物在尿液中的浓度很低,仅为纳克/毫升,这证明了全面探索暴露组所需的高灵敏度。此外,我们还发现了以前未报道过的吸烟生物标志物,如 2,6-二甲氧基苯酚和 7-甲基-1-萘酚葡萄糖醛酸苷。
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Development of an LC-HRMS non-targeted method for comprehensive profiling of the exposome of nicotine and tobacco product users – A showcase for cigarette smokers
The global prevalence of electronic cigarettes, heated tobacco products, and other smokeless alternatives has grown significantly in the last ten years. These products have been suggested as combustion-free alternatives for conventional tobacco products like cigarettes, aiming to reduce the negative health impacts associated with smoking. However, the impact of those products on the health and safety of the general population are still unclear, as the absolute exposure from those products has not been thoroughly studied, yet. In this project, a non-targeted LC-HRMS method was developed comprising four different analytical modes for the investigation of the exposure profile in urine of the product users. The method is characterized by its high sensitivity and reproducibility, as shown during method validation. As a proof of concept, we first applied this method to detect significant differences in biomarkers of exposure (BoEs) between smokers and non-smokers. We observed a total of 171 BoEs significantly elevated in smokers, including several well-known biomarkers of smoke exposure like nicotine and its metabolites, mercapturic acid derivatives, and phenolic compounds. Some of the detected biomarkers are present at low ng/mL concentrations in urine, proving the high sensitivity needed for a holistic exploration of the exposome. Moreover, we were able to identify BoEs that have not been reported previously for smoking, such as 2,6-dimethoxyphenol and 7-methyl-1-naphthol glucuronide.
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来源期刊
Journal of Chromatography B
Journal of Chromatography B 医学-分析化学
CiteScore
5.60
自引率
3.30%
发文量
306
审稿时长
44 days
期刊介绍: The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.
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