Tian Wang , Zhenjie Zhang , Fei Qiao , Fuchang Yu , Bo Jing , Junqiang Li , Meng Qi
{"title":"中国南疆规模养殖场绵羊中杜氏贾第鞭毛虫的流行率和遗传多样性。","authors":"Tian Wang , Zhenjie Zhang , Fei Qiao , Fuchang Yu , Bo Jing , Junqiang Li , Meng Qi","doi":"10.1016/j.actatropica.2024.107428","DOIUrl":null,"url":null,"abstract":"<div><div><em>Giardia duodenalis</em> is a significant zoonotic parasite. In this study, 767 fresh fecal samples were collected randomly from six large-scale sheep farms in Southern Xinjiang, China. Initially, <em>G. duodenalis</em> was screened using PCR at the SSU rRNA gene. Positive samples then underwent PCR amplification at the <em>bg, gdh</em>, and <em>tpi</em> genes. The prevalence of <em>G. duodenalis</em> in sheep was 17.5% (134/767), with the highest prevalence observed in the 3–6 months age group at 26.8% (56/209) and the lowest in the over 12 months age group at 6.8% (14/205). Among the 134 positive samples, only Assemblage E was identified. A total of 106, 92, and 98 sequences of <em>G. duodenalis</em> were obtained at the <em>gdh, tpi</em>, and <em>bg</em> genes, respectively. Fourteen isolates of <em>G. duodenalis</em> were successfully amplified at all three genes, resulting in nine <em>G. duodenalis</em> multilocus genotypes (MLG) named MLG E1-MLG E14, indicating high genetic diversity. In conclusion, <em>G. duodenalis</em> infection in sheep from large-scale farms is common in Southern Xinjiang, China, showing geographical regional distributions and genetic diversity.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence and genetic diversity of Giardia duodenalis in sheep from large-scale farms in Southern Xinjiang, China\",\"authors\":\"Tian Wang , Zhenjie Zhang , Fei Qiao , Fuchang Yu , Bo Jing , Junqiang Li , Meng Qi\",\"doi\":\"10.1016/j.actatropica.2024.107428\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Giardia duodenalis</em> is a significant zoonotic parasite. In this study, 767 fresh fecal samples were collected randomly from six large-scale sheep farms in Southern Xinjiang, China. Initially, <em>G. duodenalis</em> was screened using PCR at the SSU rRNA gene. Positive samples then underwent PCR amplification at the <em>bg, gdh</em>, and <em>tpi</em> genes. The prevalence of <em>G. duodenalis</em> in sheep was 17.5% (134/767), with the highest prevalence observed in the 3–6 months age group at 26.8% (56/209) and the lowest in the over 12 months age group at 6.8% (14/205). Among the 134 positive samples, only Assemblage E was identified. A total of 106, 92, and 98 sequences of <em>G. duodenalis</em> were obtained at the <em>gdh, tpi</em>, and <em>bg</em> genes, respectively. Fourteen isolates of <em>G. duodenalis</em> were successfully amplified at all three genes, resulting in nine <em>G. duodenalis</em> multilocus genotypes (MLG) named MLG E1-MLG E14, indicating high genetic diversity. In conclusion, <em>G. duodenalis</em> infection in sheep from large-scale farms is common in Southern Xinjiang, China, showing geographical regional distributions and genetic diversity.</div></div>\",\"PeriodicalId\":7240,\"journal\":{\"name\":\"Acta tropica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta tropica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0001706X24003097\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta tropica","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0001706X24003097","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Prevalence and genetic diversity of Giardia duodenalis in sheep from large-scale farms in Southern Xinjiang, China
Giardia duodenalis is a significant zoonotic parasite. In this study, 767 fresh fecal samples were collected randomly from six large-scale sheep farms in Southern Xinjiang, China. Initially, G. duodenalis was screened using PCR at the SSU rRNA gene. Positive samples then underwent PCR amplification at the bg, gdh, and tpi genes. The prevalence of G. duodenalis in sheep was 17.5% (134/767), with the highest prevalence observed in the 3–6 months age group at 26.8% (56/209) and the lowest in the over 12 months age group at 6.8% (14/205). Among the 134 positive samples, only Assemblage E was identified. A total of 106, 92, and 98 sequences of G. duodenalis were obtained at the gdh, tpi, and bg genes, respectively. Fourteen isolates of G. duodenalis were successfully amplified at all three genes, resulting in nine G. duodenalis multilocus genotypes (MLG) named MLG E1-MLG E14, indicating high genetic diversity. In conclusion, G. duodenalis infection in sheep from large-scale farms is common in Southern Xinjiang, China, showing geographical regional distributions and genetic diversity.
期刊介绍:
Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.