中国南疆规模养殖场绵羊中杜氏贾第鞭毛虫的流行率和遗传多样性。

IF 2.1 3区 医学 Q2 PARASITOLOGY Acta tropica Pub Date : 2024-10-10 DOI:10.1016/j.actatropica.2024.107428
Tian Wang , Zhenjie Zhang , Fei Qiao , Fuchang Yu , Bo Jing , Junqiang Li , Meng Qi
{"title":"中国南疆规模养殖场绵羊中杜氏贾第鞭毛虫的流行率和遗传多样性。","authors":"Tian Wang ,&nbsp;Zhenjie Zhang ,&nbsp;Fei Qiao ,&nbsp;Fuchang Yu ,&nbsp;Bo Jing ,&nbsp;Junqiang Li ,&nbsp;Meng Qi","doi":"10.1016/j.actatropica.2024.107428","DOIUrl":null,"url":null,"abstract":"<div><div><em>Giardia duodenalis</em> is a significant zoonotic parasite. In this study, 767 fresh fecal samples were collected randomly from six large-scale sheep farms in Southern Xinjiang, China. Initially, <em>G. duodenalis</em> was screened using PCR at the SSU rRNA gene. Positive samples then underwent PCR amplification at the <em>bg, gdh</em>, and <em>tpi</em> genes. The prevalence of <em>G. duodenalis</em> in sheep was 17.5% (134/767), with the highest prevalence observed in the 3–6 months age group at 26.8% (56/209) and the lowest in the over 12 months age group at 6.8% (14/205). Among the 134 positive samples, only Assemblage E was identified. A total of 106, 92, and 98 sequences of <em>G. duodenalis</em> were obtained at the <em>gdh, tpi</em>, and <em>bg</em> genes, respectively. Fourteen isolates of <em>G. duodenalis</em> were successfully amplified at all three genes, resulting in nine <em>G. duodenalis</em> multilocus genotypes (MLG) named MLG E1-MLG E14, indicating high genetic diversity. In conclusion, <em>G. duodenalis</em> infection in sheep from large-scale farms is common in Southern Xinjiang, China, showing geographical regional distributions and genetic diversity.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"260 ","pages":"Article 107428"},"PeriodicalIF":2.1000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence and genetic diversity of Giardia duodenalis in sheep from large-scale farms in Southern Xinjiang, China\",\"authors\":\"Tian Wang ,&nbsp;Zhenjie Zhang ,&nbsp;Fei Qiao ,&nbsp;Fuchang Yu ,&nbsp;Bo Jing ,&nbsp;Junqiang Li ,&nbsp;Meng Qi\",\"doi\":\"10.1016/j.actatropica.2024.107428\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Giardia duodenalis</em> is a significant zoonotic parasite. In this study, 767 fresh fecal samples were collected randomly from six large-scale sheep farms in Southern Xinjiang, China. Initially, <em>G. duodenalis</em> was screened using PCR at the SSU rRNA gene. Positive samples then underwent PCR amplification at the <em>bg, gdh</em>, and <em>tpi</em> genes. The prevalence of <em>G. duodenalis</em> in sheep was 17.5% (134/767), with the highest prevalence observed in the 3–6 months age group at 26.8% (56/209) and the lowest in the over 12 months age group at 6.8% (14/205). Among the 134 positive samples, only Assemblage E was identified. A total of 106, 92, and 98 sequences of <em>G. duodenalis</em> were obtained at the <em>gdh, tpi</em>, and <em>bg</em> genes, respectively. Fourteen isolates of <em>G. duodenalis</em> were successfully amplified at all three genes, resulting in nine <em>G. duodenalis</em> multilocus genotypes (MLG) named MLG E1-MLG E14, indicating high genetic diversity. In conclusion, <em>G. duodenalis</em> infection in sheep from large-scale farms is common in Southern Xinjiang, China, showing geographical regional distributions and genetic diversity.</div></div>\",\"PeriodicalId\":7240,\"journal\":{\"name\":\"Acta tropica\",\"volume\":\"260 \",\"pages\":\"Article 107428\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta tropica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0001706X24003097\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta tropica","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0001706X24003097","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

十二指肠贾第虫是一种重要的人畜共患寄生虫。本研究从中国南疆的 6 个大型养羊场随机采集了 767 份新鲜粪便样本。首先,通过 SSU rRNA 基因的 PCR 对十二指肠弧菌进行筛查。然后对阳性样本进行 bg、ghdh 和 tpi 基因的 PCR 扩增。绵羊的十二指肠球菌感染率为 17.5%(134/767),其中 3-6 月龄组感染率最高,为 26.8%(56/209),12 月龄以上组感染率最低,为 6.8%(14/205)。在 134 个阳性样本中,只发现了 E 组合。在 gdh、tpi 和 bg 基因上分别获得了 106、92 和 98 个 G. duodenalis 序列。在所有三个基因上都成功扩增出了 14 个 G. duodenalis 分离物,从而产生了 9 个 G. duodenalis 多焦点基因型(MLG),命名为 MLG E1-MLG E14,表明遗传多样性很高。总之,中国南疆地区规模化养殖场的绵羊普遍感染了十二指肠杆菌,并呈现出地理区域分布和遗传多样性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Prevalence and genetic diversity of Giardia duodenalis in sheep from large-scale farms in Southern Xinjiang, China
Giardia duodenalis is a significant zoonotic parasite. In this study, 767 fresh fecal samples were collected randomly from six large-scale sheep farms in Southern Xinjiang, China. Initially, G. duodenalis was screened using PCR at the SSU rRNA gene. Positive samples then underwent PCR amplification at the bg, gdh, and tpi genes. The prevalence of G. duodenalis in sheep was 17.5% (134/767), with the highest prevalence observed in the 3–6 months age group at 26.8% (56/209) and the lowest in the over 12 months age group at 6.8% (14/205). Among the 134 positive samples, only Assemblage E was identified. A total of 106, 92, and 98 sequences of G. duodenalis were obtained at the gdh, tpi, and bg genes, respectively. Fourteen isolates of G. duodenalis were successfully amplified at all three genes, resulting in nine G. duodenalis multilocus genotypes (MLG) named MLG E1-MLG E14, indicating high genetic diversity. In conclusion, G. duodenalis infection in sheep from large-scale farms is common in Southern Xinjiang, China, showing geographical regional distributions and genetic diversity.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Acta tropica
Acta tropica 医学-寄生虫学
CiteScore
5.40
自引率
11.10%
发文量
383
审稿时长
37 days
期刊介绍: Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.
期刊最新文献
Habu vipers (Protobothrops flavoviridis) are an overlooked but important reservoir of the zoonotic tick Amblyomma testudinarium (Acari: Ixodidae) in subtropical Asia. A methodological proposal to estimate the total abundance of immature mosquitoes in discarded tyres: Aedes aegypti and Culex pipiens as study cases. Harnessing computational and experimental approaches to identify potent hits against Leishmania donovani sterol C-24 methyltransferase from ChemBridge library. Evidence of endogenous Non-retroviral RNA Virus Sequences into the genome and transcriptome of the malaria vector Anopheles darlingi. Insights into Leptospira spp. infection in pet cats in Croatia: Clinical, serological and molecular findings with emphasis on the potential important role of serogroup Pomona
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1