研磨的沙棘草粉通过抑制 Wnt/β-catenin 信号转导和 Nrf2 抑制 AOM/DSS 诱导的结肠癌

IF 3.5 3区医学 Q2 CHEMISTRY, MULTIDISCIPLINARY Pharmaceutical Research Pub Date : 2024-10-21 DOI:10.1007/s11095-024-03784-1

Yeonoh Cho, Ji Hyeon Cha, Yujin Hwang, Hee-Taik Kang, Jong Hun Lee

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引用次数: 0

摘要

目的：本研究旨在评估益生元和益生菌对AOM/DSS诱导的小鼠模型中结直肠癌（CRC）进展的影响：方法：在AOM/DSS诱导的小鼠模型中，治疗组接受草本酵母作为益生菌（PRE）或与植物乳杆菌联合作为益生菌（PRO）。采用免疫组织化学（IHC）方法对 PCNA、Ki-67、β-catenin、c-Myc 和 Nrf2 进行了评估。根据息肉的大小进行分类，评估其对息肉形成和发展的影响：结果：与AOM/DSS诱导的对照组相比，PRE和PRO治疗均显著减少了大息肉的形成。IHC分析表明，PRE和PRO组细胞增殖的生物标志物表达减少，特别是PCNA、Ki-67、β-catenin和c-Myc的染色减少，表明Wnt信号下调，细胞增殖受到抑制。Nrf2 表达的减少凸显了干扰癌细胞防御功能的治疗所产生的影响。值得注意的是，PRE 组和 PRO 组的结果没有明显差异，这表明益生元具有抗癌作用：研究表明，益生菌 S. herbacea 能有效抑制 CRC 的进展，与益生菌结合使用能带来的额外益处有限。这些发现强调了益生元对 CRC 的治疗潜力。

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Ground Salicornia herbacea Powder Suppresses AOM/DSS-induced Colon Cancer by Inhibiting Wnt/β-catenin Signaling and Nrf2.

Purpose: This study aims to evaluate the effects of prebiotics and probiotics on colorectal cancer (CRC) progression in an AOM/DSS-induced mouse model.

Methods: In AOM/DSS-induced mouse model, treatment groups received either S. herbacea as a prebiotic (PRE) or in combination with Lactobacillus plantarum as a probiotic (PRO). PCNA, Ki-67, β-catenin, c-Myc, and Nrf2 were evaluated using immunohistochemistry (IHC). The impact on polyp formation and progression was assessed by categorizing polyps according to their size.

Results: Both PRE and PRO treatments resulted in a significant reduction in large polyp formation when compared to AOM/DSS induced control group. IHC analyses demonstrated reduced biomarker expression for cell proliferation in PRE and PRO groups, specifically showing decreased staining for PCNA, Ki-67, β-catenin, and c-Myc, indicating downregulation of Wnt signaling and suppressed cell proliferation. Reduced Nrf2 expression highlights the impact of treatments interfering with cancer cell defenses. Notably, there were no significant differences in the outcomes between PRE and PRO groups, suggesting that prebiotics show anticancer effects.

Conclusion: The study suggests that S. herbacea, a prebiotic, effectively suppresses CRC progression, with limited additional benefits from combining with probiotics. These findings underscore the therapeutic potential of prebiotics in CRC.

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来源期刊

Pharmaceutical Research 医学-化学综合

CiteScore

6.60

自引率

5.40%

发文量

276

审稿时长

3.4 months

期刊介绍： Pharmaceutical Research, an official journal of the American Association of Pharmaceutical Scientists, is committed to publishing novel research that is mechanism-based, hypothesis-driven and addresses significant issues in drug discovery, development and regulation. Current areas of interest include, but are not limited to: -(pre)formulation engineering and processing- computational biopharmaceutics- drug delivery and targeting- molecular biopharmaceutics and drug disposition (including cellular and molecular pharmacology)- pharmacokinetics, pharmacodynamics and pharmacogenetics. Research may involve nonclinical and clinical studies, and utilize both in vitro and in vivo approaches. Studies on small drug molecules, pharmaceutical solid materials (including biomaterials, polymers and nanoparticles) biotechnology products (including genes, peptides, proteins and vaccines), and genetically engineered cells are welcome.