加强猫科动物保护：探索体外培养基对家猫囊胚生产的影响。

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI:10.1111/rda.14645

Ana Munoz-Maceda, Andrea Priego-Gonzalez, Carolina Núñez-Puente, Dimitrios Rizos, Joaquín Cerdeira-Lozano, Ana Sanchez-Rodriguez, Eduardo R S Roldan, Maria Jesus Sánchez-Calabuig

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引用次数: 0

摘要

这项研究调查了野生猫科动物保护辅助生殖技术的优化情况，重点是以家猫为模式物种的体外程序。研究评估了三种不同体外培养基对囊胚形成的影响。收集和处理卵细胞和精子，然后进行体外受精和培养。结果表明，各组的囊胚率相似（方差分析，p > .05），均超过 16%。在证明这些技术的潜力的同时，还需要进一步调查以评估胚胎质量，从而完善最佳方案及其在猫科动物保护工作中的适用性。

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Enhancing felid conservation: Exploring the impact of in vitro culture media on domestic cat blastocyst production.

This study investigated the optimization of assisted reproductive techniques for wild felid conservation, focusing on in vitro procedures using the domestic cat as a model species. The research evaluated the impact of three different in vitro culture media on blastocyst formation. Oocytes and spermatozoa were collected and processed, followed by in vitro fertilization and culture. Results returned a similar blastocyst rate (ANOVA, p > .05), over 16% across all groups. While demonstrating the potential of these techniques, further investigations are warranted to evaluate embryo quality to refine optimal protocols and their applicability in felid conservation efforts.

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.