{"title":"在光照条件下评估核黄素、纳米姜黄素和过氧化氢：减少成熟牙齿生物膜和釉质矿物质流失。","authors":"Shima Afrasiabi , Ahmed Qays Khudhur AL． Gburi , Ladan Ranjbar Omrani , Nasim Chiniforush , Zohreh Moradi","doi":"10.1016/j.pdpdt.2024.104379","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Biofilms are a potential harbor for many microorganisms. The aim of this study was to test the efficacy of riboflavin (Rib), nano-micelle curcumin (NC), and hydrogen peroxide (HP), alone and in combination with the respective light (light-emitting diode (LED) or 980 nm diode laser) on the reduction of <em>Streptococcus mutans</em> and <em>Lactobacillus acidophilus</em> dual-species biofilms and their effect on the enamel mineral loss.</div></div><div><h3>Materials and methods</h3><div>The biofilms were formed on saliva-coated enamel slabs. Then, the biofilms were treated with antimicrobial photodynamic therapy (PDT) based on LED, Rib, and NC photosensitizers and with HP also based on a 980 nm diode laser (<em>n</em> = 8 per group). A crystal violet assay was performed to determine the reduction of the dual-species biofilms. The enamel slabs were analyzed for calcium and phosphorus content by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX).</div></div><div><h3>Results</h3><div>While HP-PDT showed a reduction of 37% (<em>p</em> < 0.001), PDT with NC resulted in an even greater reduction of dual-species biofilms (40%, <em>p</em> < 0.001) than HP- and Rib-mediated PDT. In the EDX test, no significant difference was found between the control group and the treatment groups.</div></div><div><h3>Conclusions</h3><div>The use of natural photosensitizers such as NC in PDT has an effect that may be potentially important in reducing caries-causing bacteria.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"50 ","pages":"Article 104379"},"PeriodicalIF":3.1000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of riboflavin, nanocurcumin, and hydrogen peroxide under light conditions: Reduction of mature dental biofilms and enamel mineral loss\",\"authors\":\"Shima Afrasiabi , Ahmed Qays Khudhur AL． Gburi , Ladan Ranjbar Omrani , Nasim Chiniforush , Zohreh Moradi\",\"doi\":\"10.1016/j.pdpdt.2024.104379\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Biofilms are a potential harbor for many microorganisms. The aim of this study was to test the efficacy of riboflavin (Rib), nano-micelle curcumin (NC), and hydrogen peroxide (HP), alone and in combination with the respective light (light-emitting diode (LED) or 980 nm diode laser) on the reduction of <em>Streptococcus mutans</em> and <em>Lactobacillus acidophilus</em> dual-species biofilms and their effect on the enamel mineral loss.</div></div><div><h3>Materials and methods</h3><div>The biofilms were formed on saliva-coated enamel slabs. Then, the biofilms were treated with antimicrobial photodynamic therapy (PDT) based on LED, Rib, and NC photosensitizers and with HP also based on a 980 nm diode laser (<em>n</em> = 8 per group). A crystal violet assay was performed to determine the reduction of the dual-species biofilms. The enamel slabs were analyzed for calcium and phosphorus content by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX).</div></div><div><h3>Results</h3><div>While HP-PDT showed a reduction of 37% (<em>p</em> < 0.001), PDT with NC resulted in an even greater reduction of dual-species biofilms (40%, <em>p</em> < 0.001) than HP- and Rib-mediated PDT. In the EDX test, no significant difference was found between the control group and the treatment groups.</div></div><div><h3>Conclusions</h3><div>The use of natural photosensitizers such as NC in PDT has an effect that may be potentially important in reducing caries-causing bacteria.</div></div>\",\"PeriodicalId\":20141,\"journal\":{\"name\":\"Photodiagnosis and Photodynamic Therapy\",\"volume\":\"50 \",\"pages\":\"Article 104379\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2024-10-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Photodiagnosis and Photodynamic Therapy\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1572100024004162\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photodiagnosis and Photodynamic Therapy","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1572100024004162","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ONCOLOGY","Score":null,"Total":0}
Evaluation of riboflavin, nanocurcumin, and hydrogen peroxide under light conditions: Reduction of mature dental biofilms and enamel mineral loss
Background
Biofilms are a potential harbor for many microorganisms. The aim of this study was to test the efficacy of riboflavin (Rib), nano-micelle curcumin (NC), and hydrogen peroxide (HP), alone and in combination with the respective light (light-emitting diode (LED) or 980 nm diode laser) on the reduction of Streptococcus mutans and Lactobacillus acidophilus dual-species biofilms and their effect on the enamel mineral loss.
Materials and methods
The biofilms were formed on saliva-coated enamel slabs. Then, the biofilms were treated with antimicrobial photodynamic therapy (PDT) based on LED, Rib, and NC photosensitizers and with HP also based on a 980 nm diode laser (n = 8 per group). A crystal violet assay was performed to determine the reduction of the dual-species biofilms. The enamel slabs were analyzed for calcium and phosphorus content by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX).
Results
While HP-PDT showed a reduction of 37% (p < 0.001), PDT with NC resulted in an even greater reduction of dual-species biofilms (40%, p < 0.001) than HP- and Rib-mediated PDT. In the EDX test, no significant difference was found between the control group and the treatment groups.
Conclusions
The use of natural photosensitizers such as NC in PDT has an effect that may be potentially important in reducing caries-causing bacteria.
期刊介绍：
Photodiagnosis and Photodynamic Therapy is an international journal for the dissemination of scientific knowledge and clinical developments of Photodiagnosis and Photodynamic Therapy in all medical specialties. The journal publishes original articles, review articles, case presentations, "how-to-do-it" articles, Letters to the Editor, short communications and relevant images with short descriptions. All submitted material is subject to a strict peer-review process.
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