独特耦合反应引发的活细胞多巴胺荧光成像

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL Analytica Chimica Acta Pub Date : 2024-10-22 DOI:10.1016/j.aca.2024.343358

Shaofei Fu , Jianwen Qiu , Biaofeng Gu , Yong Gao

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引用次数: 0

摘要

背景多巴胺（DA）是哺乳动物大脑中一种重要的神经递质和儿茶酚胺分子。它在感知、认知、中枢神经系统调节和激素分泌中起着至关重要的作用。结果在这项研究中，我们通过将 DA 检测反应与 HClO 氧化反应耦合，在生理 pH 条件下实现了有效的 DA 特异荧光生成。该方法已被用于细胞中 DA 的检测，以及炎症和抑郁细胞模型中 DA 水平的可视化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Fluorescence imaging of dopamine in living cells triggered by unique coupling reactions

Background

Dopamine (DA) is a significant neurotransmitter and catecholamine molecule in the mammalian brain. It plays a crucial role in perception, cognition, central nervous system regulation, and hormone secretion. The detection of DA levels in living systems is a vital aspect of the research and early diagnosis of neurological disorders.

Results

In this study, we achieved an effective fluorescence generation specific to DA under physiological pH conditions by coupling the DA detection reaction to the HClO oxidation reaction. This method has been used for the detection of DA in cells, as well as for the visualization of DA levels in cellular models of inflammation and depression.

Significance

This is the first time that utilizes an organic fluorescent molecular probe to detect DA in living cells.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.