智利本地昆虫病原真菌的分离和鉴定及其控制铃木果蝇的潜力。

IF 3.6 3区 生物学 Q1 ZOOLOGY Journal of invertebrate pathology Pub Date : 2024-11-01 DOI:10.1016/j.jip.2024.108223
Cristian Montalva , Cristian González , Cecilia Ruiz , Isabel Vives , Andrea Díaz , Luis Devotto , Juscelino Rodrigues , Luiz F.N. Rocha , Javiera Manríquez , Tania Vásquez , Richard A. Humber , Christian Luz
{"title":"智利本地昆虫病原真菌的分离和鉴定及其控制铃木果蝇的潜力。","authors":"Cristian Montalva ,&nbsp;Cristian González ,&nbsp;Cecilia Ruiz ,&nbsp;Isabel Vives ,&nbsp;Andrea Díaz ,&nbsp;Luis Devotto ,&nbsp;Juscelino Rodrigues ,&nbsp;Luiz F.N. Rocha ,&nbsp;Javiera Manríquez ,&nbsp;Tania Vásquez ,&nbsp;Richard A. Humber ,&nbsp;Christian Luz","doi":"10.1016/j.jip.2024.108223","DOIUrl":null,"url":null,"abstract":"<div><div><em>Drosophila suzukii</em> is an invasive pest of berries and other soft-skinned fruits, was first detected in Chile in 2017, and has since spread over 2,800 km from north to south. Sustainable control of the spotted-wing drosophila (SWD) is essential due the negative attitude of the consumers toward the excessive use of insecticides. During a survey in Chile for biological control agents, thirty-two isolates of entomopathogenic fungi (EPF) were isolated from mycotized insects and soil samples, identified through sequence analysis, and tested against <em>D. suzukii</em> adults under laboratory conditions. The EPF identified are <em>Akanthomyces muscarius</em>, <em>Beauveria bassiana</em>, <em>Beauveria pseudobassiana</em>, <em>Clonostachys rosea</em>, <em>Metarhizium alvesii</em>, <em>Metarhizium brunneum</em> and <em>Metarhizium robertsii</em>. Six isolates caused 100 % of mortality of <em>D. suzukii</em> adults within ten days after the initial exposition to conidia; these included four <em>B</em>. <em>bassiana</em> isolates (LSB 110, LSB 114, LSB 122 and LSB 125), one <em>M</em>. <em>robertsii</em> isolate (LSB 115) and one <em>M</em>. <em>brunneum</em> isolate (LSB 127). LSB 122 and LSB 125 induced the shortest lethal time (LT<sub>50</sub> 4 and 4.2 days, respectively), while <em>Akanthomyces</em> and <em>Clonostachys</em> caused ≤ 36 % cumulative mortality of the adults at 10 days of exposure. These results indicate that isolates of <em>B. bassiana</em>, <em>M. robertsii</em> and <em>M. brunneum</em> have significant potential as microbial control agents against <em>D. suzukii</em> adults. This study marks a critical step forward in identifying and validating native entomopathogenic fungi in Chile for sustainable pest management. Future work will focus on further testing these isolates under laboratory, semi-field and field conditions to optimize their application in real-world agricultural settings.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"207 ","pages":"Article 108223"},"PeriodicalIF":3.6000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation and identification of native Chilean entomopathogenic fungi and their potential for the control of Drosophila suzukii\",\"authors\":\"Cristian Montalva ,&nbsp;Cristian González ,&nbsp;Cecilia Ruiz ,&nbsp;Isabel Vives ,&nbsp;Andrea Díaz ,&nbsp;Luis Devotto ,&nbsp;Juscelino Rodrigues ,&nbsp;Luiz F.N. Rocha ,&nbsp;Javiera Manríquez ,&nbsp;Tania Vásquez ,&nbsp;Richard A. Humber ,&nbsp;Christian Luz\",\"doi\":\"10.1016/j.jip.2024.108223\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Drosophila suzukii</em> is an invasive pest of berries and other soft-skinned fruits, was first detected in Chile in 2017, and has since spread over 2,800 km from north to south. Sustainable control of the spotted-wing drosophila (SWD) is essential due the negative attitude of the consumers toward the excessive use of insecticides. During a survey in Chile for biological control agents, thirty-two isolates of entomopathogenic fungi (EPF) were isolated from mycotized insects and soil samples, identified through sequence analysis, and tested against <em>D. suzukii</em> adults under laboratory conditions. The EPF identified are <em>Akanthomyces muscarius</em>, <em>Beauveria bassiana</em>, <em>Beauveria pseudobassiana</em>, <em>Clonostachys rosea</em>, <em>Metarhizium alvesii</em>, <em>Metarhizium brunneum</em> and <em>Metarhizium robertsii</em>. Six isolates caused 100 % of mortality of <em>D. suzukii</em> adults within ten days after the initial exposition to conidia; these included four <em>B</em>. <em>bassiana</em> isolates (LSB 110, LSB 114, LSB 122 and LSB 125), one <em>M</em>. <em>robertsii</em> isolate (LSB 115) and one <em>M</em>. <em>brunneum</em> isolate (LSB 127). LSB 122 and LSB 125 induced the shortest lethal time (LT<sub>50</sub> 4 and 4.2 days, respectively), while <em>Akanthomyces</em> and <em>Clonostachys</em> caused ≤ 36 % cumulative mortality of the adults at 10 days of exposure. These results indicate that isolates of <em>B. bassiana</em>, <em>M. robertsii</em> and <em>M. brunneum</em> have significant potential as microbial control agents against <em>D. suzukii</em> adults. This study marks a critical step forward in identifying and validating native entomopathogenic fungi in Chile for sustainable pest management. Future work will focus on further testing these isolates under laboratory, semi-field and field conditions to optimize their application in real-world agricultural settings.</div></div>\",\"PeriodicalId\":16296,\"journal\":{\"name\":\"Journal of invertebrate pathology\",\"volume\":\"207 \",\"pages\":\"Article 108223\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of invertebrate pathology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022201124001666\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of invertebrate pathology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022201124001666","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

斑翅果蝇是浆果和其他软皮水果的入侵害虫,2017 年首次在智利发现,此后从北到南蔓延了 2800 公里。由于消费者对过度使用杀虫剂持消极态度,因此对斑翅果蝇(SWD)的可持续控制至关重要。在智利进行的一项生物控制剂调查中,从霉菌化昆虫和土壤样本中分离出 32 个昆虫病原真菌(EPF)分离物,通过序列分析进行鉴定,并在实验室条件下对苏氏点翅夜蛾成虫进行了测试。鉴定出的 EPF 包括 Akanthomyces muscarius、Beauveria bassiana、Beauveria pseudobassiana、Clonostachys rosea、Metarhizium alvesii、Metarhizium brunneum 和 Metarhizium robertsii。六种分离物在最初接触分生孢子后的十天内导致 D. suzukii 成虫 100%死亡;其中包括四种 B. bassiana 分离物(LSB 110、LSB 114、LSB 122 和 LSB 125)、一种 M. robertsii 分离物(LSB 115)和一种 M. brunneum 分离物(LSB 127)。LSB 122 和 LSB 125 诱导的致死时间最短(LT50 分别为 4 天和 4.2 天),而 Akanthomyces 和 Clonostachys 在暴露 10 天后造成的成虫累积死亡率≤36%。这些结果表明,B. bassiana、M. robertsii和M. brunneum的分离物作为微生物控制剂,对防治D. suzukii成虫具有重大潜力。这项研究标志着智利在鉴定和验证本地昆虫病原真菌以进行可持续害虫管理方面迈出了关键一步。未来的工作重点是在实验室、半田间和田间条件下进一步测试这些分离物,以优化它们在实际农业环境中的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Isolation and identification of native Chilean entomopathogenic fungi and their potential for the control of Drosophila suzukii
Drosophila suzukii is an invasive pest of berries and other soft-skinned fruits, was first detected in Chile in 2017, and has since spread over 2,800 km from north to south. Sustainable control of the spotted-wing drosophila (SWD) is essential due the negative attitude of the consumers toward the excessive use of insecticides. During a survey in Chile for biological control agents, thirty-two isolates of entomopathogenic fungi (EPF) were isolated from mycotized insects and soil samples, identified through sequence analysis, and tested against D. suzukii adults under laboratory conditions. The EPF identified are Akanthomyces muscarius, Beauveria bassiana, Beauveria pseudobassiana, Clonostachys rosea, Metarhizium alvesii, Metarhizium brunneum and Metarhizium robertsii. Six isolates caused 100 % of mortality of D. suzukii adults within ten days after the initial exposition to conidia; these included four B. bassiana isolates (LSB 110, LSB 114, LSB 122 and LSB 125), one M. robertsii isolate (LSB 115) and one M. brunneum isolate (LSB 127). LSB 122 and LSB 125 induced the shortest lethal time (LT50 4 and 4.2 days, respectively), while Akanthomyces and Clonostachys caused ≤ 36 % cumulative mortality of the adults at 10 days of exposure. These results indicate that isolates of B. bassiana, M. robertsii and M. brunneum have significant potential as microbial control agents against D. suzukii adults. This study marks a critical step forward in identifying and validating native entomopathogenic fungi in Chile for sustainable pest management. Future work will focus on further testing these isolates under laboratory, semi-field and field conditions to optimize their application in real-world agricultural settings.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
6.10
自引率
5.90%
发文量
94
审稿时长
1 months
期刊介绍: The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.
期刊最新文献
Bombyx mori PAT4 gene inhibits BmNPV infection and replication through autophagy. A histopathological guide for the social spider Stegodyphus dumicola. Inheritance of Cry2Ab2 resistance in two Helicoverpa zea(Lepidoptera: Noctuidae) populations resistant to single- and dual-Bacillus thuringiensis proteins. Multiple diptericins of the black soldier fly (Hermetia illucens) differentially respond to bacterial challenges. Identification of Albopleistophora grylli n. gen. n. sp. (Microsporidia) and its impact on crickets (Gryllus spp.) in food-and-feed culture systems.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1