Maxim S. Kokoulin , Vlada S. Belova , Lyudmila A. Romanenko
{"title":"细菌解离对脂多糖结构的影响：对海洋细菌 Pseudoalteromonas agarivorans KMM 232（O-form）O 型多糖的研究。","authors":"Maxim S. Kokoulin , Vlada S. Belova , Lyudmila A. Romanenko","doi":"10.1016/j.carres.2024.109300","DOIUrl":null,"url":null,"abstract":"<div><div>The lipopolysaccharide (LPS) was obtained from a bacterium <em>Pseudoalteromonas agarivorans</em> KMM 232 (O-form) isolated from a seawater sample collected at a depth of 500 m. The O-polysaccharide (OPS) was isolated by mild acid degradation of the LPS and studied by chemical methods along with 1D and 2D <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy, including <sup>1</sup>H,<sup>1</sup>H COSY, <sup>1</sup>H,<sup>1</sup>H TOCSY, <sup>1</sup>H,<sup>1</sup>H ROESY and <sup>1</sup>H,<sup>13</sup>C HSQC, and <sup>1</sup>H,<sup>13</sup>C HMBC experiments. The following new structure of the OPS from <em>P. agarivorans</em> KMM 232 (O-form) containing 2-acetamido-2-deoxy-<span>d</span>-glucose (D-GlcNAc), <span>d</span>-glucose (D-Glc), <span>d</span>-glucuronic acid (D-GlcA), 4,6-<em>O</em>-[(<em>R</em>)-1-carboxyethylidene]-<span>d</span>-galactose [D-Gal<em>p</em>4,6 (<em>R</em>-Pyr)] and two residues of <span>d</span>-galactose (D-Gal) was established:</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"545 ","pages":"Article 109300"},"PeriodicalIF":2.4000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of bacterial dissociation on lipopolysaccharide structure: A study of O-polysaccharide from the marine bacterium Pseudoalteromonas agarivorans KMM 232 (O-form)\",\"authors\":\"Maxim S. Kokoulin , Vlada S. Belova , Lyudmila A. Romanenko\",\"doi\":\"10.1016/j.carres.2024.109300\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The lipopolysaccharide (LPS) was obtained from a bacterium <em>Pseudoalteromonas agarivorans</em> KMM 232 (O-form) isolated from a seawater sample collected at a depth of 500 m. The O-polysaccharide (OPS) was isolated by mild acid degradation of the LPS and studied by chemical methods along with 1D and 2D <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy, including <sup>1</sup>H,<sup>1</sup>H COSY, <sup>1</sup>H,<sup>1</sup>H TOCSY, <sup>1</sup>H,<sup>1</sup>H ROESY and <sup>1</sup>H,<sup>13</sup>C HSQC, and <sup>1</sup>H,<sup>13</sup>C HMBC experiments. The following new structure of the OPS from <em>P. agarivorans</em> KMM 232 (O-form) containing 2-acetamido-2-deoxy-<span>d</span>-glucose (D-GlcNAc), <span>d</span>-glucose (D-Glc), <span>d</span>-glucuronic acid (D-GlcA), 4,6-<em>O</em>-[(<em>R</em>)-1-carboxyethylidene]-<span>d</span>-galactose [D-Gal<em>p</em>4,6 (<em>R</em>-Pyr)] and two residues of <span>d</span>-galactose (D-Gal) was established:</div></div>\",\"PeriodicalId\":9415,\"journal\":{\"name\":\"Carbohydrate Research\",\"volume\":\"545 \",\"pages\":\"Article 109300\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Carbohydrate Research\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0008621524002799\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Research","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0008621524002799","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Effect of bacterial dissociation on lipopolysaccharide structure: A study of O-polysaccharide from the marine bacterium Pseudoalteromonas agarivorans KMM 232 (O-form)
The lipopolysaccharide (LPS) was obtained from a bacterium Pseudoalteromonas agarivorans KMM 232 (O-form) isolated from a seawater sample collected at a depth of 500 m. The O-polysaccharide (OPS) was isolated by mild acid degradation of the LPS and studied by chemical methods along with 1D and 2D 1H and 13C NMR spectroscopy, including 1H,1H COSY, 1H,1H TOCSY, 1H,1H ROESY and 1H,13C HSQC, and 1H,13C HMBC experiments. The following new structure of the OPS from P. agarivorans KMM 232 (O-form) containing 2-acetamido-2-deoxy-d-glucose (D-GlcNAc), d-glucose (D-Glc), d-glucuronic acid (D-GlcA), 4,6-O-[(R)-1-carboxyethylidene]-d-galactose [D-Galp4,6 (R-Pyr)] and two residues of d-galactose (D-Gal) was established:
期刊介绍：
Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects.
Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence.
Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".
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