用荧光显微镜研究从 Jania rubens 中合成的锌纳米颗粒对 MCF-7 细胞系的细胞毒性影响。

IF 3.2 4区 化学 Q2 CHEMISTRY, ANALYTICAL Luminescence Pub Date : 2024-10-31 DOI:10.1002/bio.70007
Duraisamy Rajasekaran, Pomman Sathishkumar, Mani Neelakandan, Kannan Revathi, Jothi Vinoth Kumar, P. Srinivasan, Sandhanasamy Devanesan, Ponnusamy Selvamaleeswaran
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引用次数: 0

摘要

锌纳米粒子(ZnNPs)因其特殊功能而成为包括癌症治疗在内的许多学科的可行选择。在几种合成 ZnNP 的技术中,利用天然提取物进行生物合成是一种高效且对环境无害的方法,尤其适用于生物医学领域。在这项研究中,我们利用海洋红海藻 Jania rubens 的水提取物,创造了一种独特的生物合成技术来制造 ZnNPs。通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)观察,制得的 ZnNPs 具有独特的花朵状形态。能量色散 X 射线光谱(EDAX)和傅立叶变换红外光谱(FTIR)技术验证了 ZnNPs 的有效生产以及合成过程中生物大分子的参与。利用 MTT 试验评估了生物合成 ZnNPs 的细胞毒性作用,结果表明它们能够抑制 MCF-7 乳腺癌细胞。此外,活体/死体成像实验也验证了 ZnNPs 对 MCF-7 细胞的细胞毒性,从而支持了 MTT 实验结果。
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Fluorescent Microscopy Investigation of Cytotoxic Impact on MCF-7 Cell Line Treated With Zinc Nanoparticles Synthesized From Jania rubens

Zinc nanoparticles (ZnNPs) are a viable option in a number of disciplines, including cancer treatment, due to their special features. Among the several techniques for synthesizing ZnNP, biosynthesis with natural extracts is a highly effective and environmentally benign method, especially for uses in biomedicine. Using an aqueous extract of the marine red seaweed Jania rubens, we created a unique biosynthetic technique in this study to manufacture ZnNPs. The produced ZnNPs have a characteristic flower-like form, as seen by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The effective production of ZnNPs and the involvement of biomolecules in the synthesis process were validated by energy-dispersive X-ray spectroscopy (EDAX) and Fourier transform infrared spectroscopy (FTIR) techniques. Using the MTT assay, the cytotoxic effects of the biosynthesized ZnNPs were evaluated, indicating their ability to inhibit MCF-7 breast cancer cells. Furthermore, ZnNPs' cytotoxicity against MCF-7 cells was validated by live/dead imaging experiments, which supported the MTT results.

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来源期刊
Luminescence
Luminescence 生物-生化与分子生物学
CiteScore
5.10
自引率
13.80%
发文量
248
审稿时长
3.5 months
期刊介绍: Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.
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