Tuo Yin, Rong Xu, Ling Zhu, Xiuyao Yang, Mengjie Zhang, Xulin Li, Yinqiang Zi, Ke Wen, Ke Zhao, Hanbing Cai, Xiaozhen Liu, Hanyao Zhang
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引用次数: 0
摘要
背景:苯丙氨酸氨基转移酶(PAL)基因是一种研究较多的植物防御基因,对植物的生长、发育和抗逆性至关重要。对许多植物的 PAL 基因家族都进行过研究。柑橘是全球最重要的经济作物之一。然而,在大多数柑橘物种中,PAL 基因家族尚未得到全面研究,其生物学功能和具体的内在机制尚不清楚:结果:我们从 9 个柑橘物种中发现了 41 个 PAL 基因,并揭示了不同柑橘物种中 PAL 基因的不同进化模式。研究发现,基因复制是柑橘中 PAL 基因家族扩展的重要机制。此外,PAL 基因应对压力的能力与它们在柑橘中的进化持续时间之间存在密切联系。进化时间较短的 PAL 基因参与了更多的多种胁迫响应,而且这些具有广谱抗性的 PAL 基因都是单拷贝基因。通过进一步整合柑橘木质素和类黄酮的合成途径,我们观察到PAL基因有助于木质素和类黄酮的合成,而木质素和类黄酮能增强柑橘植物的物理防御和清除ROS的能力,从而帮助它们抵御胁迫:本研究为柑橘中的 PAL 基因家族提供了一个全面的框架,并为柑橘的抗逆机制提出了一个假设模型。本研究为进一步研究 PAL 基因在柑橘生长、发育和应对各种胁迫中的生物学功能奠定了基础。
Comparative analysis of the PAL gene family in nine citruses provides new insights into the stress resistance mechanism of Citrus species.
Background: The phenylalanine ammonia-lyase (PAL) gene, a well-studied plant defense gene, is crucial for growth, development, and stress resistance. The PAL gene family has been studied in many plants. Citrus is among the most vital cash crops worldwide. However, the PAL gene family has not been comprehensively studied in most Citrus species, and the biological functions and specific underlying mechanisms are unclear.
Results: We identified 41 PAL genes from nine Citrus species and revealed different patterns of evolution among the PAL genes in different Citrus species. Gene duplication was found to be a vital mechanism for the expansion of the PAL gene family in citrus. In addition, there was a strong correlation between the ability of PAL genes to respond to stress and their evolutionary duration in citrus. PAL genes with shorter evolutionary times were involved in more multiple stress responses, and these PAL genes with broad-spectrum resistance were all single-copy genes. By further integrating the lignin and flavonoid synthesis pathways in citrus, we observed that PAL genes contribute to the synthesis of lignin and flavonoids, which enhance the physical defense and ROS scavenging ability of citrus plants, thereby helping them withstand stress.
Conclusions: This study provides a comprehensive framework of the PAL gene family in citrus, and we propose a hypothetical model for the stress resistance mechanism in citrus. This study provides a foundation for further investigations into the biological functions of PAL genes in the growth, development, and response to various stresses in citrus.
期刊介绍:
BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics.
BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.