Weiheng Kong , Yingqi Zhao , Xiaoxia Dai , Changjun You
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引用次数: 0
摘要
人类基因组不断受到内源性和环境 DNA 损伤因子的威胁,这些损伤因子可诱发各种化学修饰的 DNA 病变,包括 8-氧代-7,8-二氢鸟嘌呤(OG)。越来越多的证据表明,OG 不仅是氧化 DNA 损伤的生物标志物,还是一种新型的表观遗传修饰,参与哺乳动物细胞中基因表达的调控。在此,我们总结了 OG 研究的最新进展,重点关注以下几点:(i) OG 在生物体内的产生机制及其在细胞中的生物学后果;(ii) 在低丰度基因组和复杂生物背景中准确鉴定 OG;(iii) OG 测序方法的发展。这些研究将有助于进一步了解 OG 诱导诱变的分子机制及其在人类发育和癌症等疾病中的潜在作用。
Methodologies for the detection and sequencing of the epigenetic-like oxidative DNA modification, 8-oxo-7,8-dihydroguanine
The human genome is constantly threatened by endogenous and environmental DNA damaging agents that can induce a variety of chemically modified DNA lesions including 8-oxo-7,8-dihydroguanine (OG). Increasing evidence has indicated that OG is not only a biomarker for oxidative DNA damage but also a novel epigenetic-like modification involved in regulation of gene expression in mammalian cells. Here we summarize the recent progress in OG research focusing on the following points: (i) the mechanism of OG production in organisms and its biological consequences in cells, (ii) the accurate identification of OG in low-abundance genomes and complex biological backgrounds, (iii) the development of OG sequencing methods. These studies will be helpful for further understanding of the molecular mechanisms of OG-induced mutagenesis and its potential roles in human development and diseases such as cancer.
期刊介绍:
The subject areas of Reviews in Mutation Research encompass the entire spectrum of the science of mutation research and its applications, with particular emphasis on the relationship between mutation and disease. Thus this section will cover advances in human genome research (including evolving technologies for mutation detection and functional genomics) with applications in clinical genetics, gene therapy and health risk assessment for environmental agents of concern.