Romane Milleville , Linda Hadjadj , Jean-Marc Rolain , Sophie Alexandra Baron
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Study of the mechanisms of colistin heteroresistance in a strain of Enterobacter cloacae by random mutagenesis
Objective
The Enterobacter cloacae complex belongs to a group in which colistin resistance is not well documented due to their frequent heteroresistance.
Methods
We investigated the molecular basis of colistin heteroresistance using genome analysis and random mutagenesis in a strain of E. cloacae.
Results
The arnA gene was truncated in the six colistin susceptible mutants we obtained. However, the mcr-9 gene was present in these mutants suggesting this gene is not responsible for colistin resistance.
Conclusions
Previous studies have highlighted the role of the two-component system PhoPQ and its negative regulator mgrB or the arnBCADTEF operon. Our results confirm the crucial role of this operon.
期刊介绍：
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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