{"title":"基于双功能 MOFs 的比率荧光平台，用于灵敏检测有机磷农药","authors":"Siying Wu , Xuehua Zhang , Wenyan Tang , Yue Zhang , Xin Lv , Yu-Ting Zhuang","doi":"10.1016/j.jfca.2024.106971","DOIUrl":null,"url":null,"abstract":"<div><div>Rapid and precise detection of organophosphorus pesticides (OPs) is of great importance for life health. Herein, a new sensing platform is developed through a dual signal amplification approach for sensitive quantification of OPs, with a bimetallic metal organic framework (UiO-66(Zr/Fe)-NH<sub>2</sub>) as peroxide-like nanozyme and fluorescent indicator. In the presence of thiocholine (TCh), the hydrolyzate of acetylthiocholine iodide catalyzed by acetylcholinesterase, the catalytic oxidization of o-phenylenediamine by UiO-66(Fe/Zr)-NH<sub>2</sub> to strong fluorescent 2,3-diaminophenazine (λ<sub>em</sub> 555 nm) is significantly inhibited. OPs-triggered acetylcholinesterase inactivation enables the renaturation of UiO-66(Fe/Zr)-NH<sub>2</sub>. Interestingly, the introduction of 1,4-dioxane further boosts the amplification of fluorescence signals. By virtue of the recovered fluorescence at 555 nm and MOF fluorescence (λ<sub>em</sub> 446 nm), the dual signal amplification-based ratiometric fluorescent platform is successfully employed for the selective recognition and sensitive detection of OPs. This work is expected to provide a facile and sensitive method for accurate OPs assay, and make a positive contribution to food safety.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106971"},"PeriodicalIF":4.0000,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A ratiometric fluorescence platform based on bifunctional MOFs for sensitive detection of organophosphorus pesticides\",\"authors\":\"Siying Wu , Xuehua Zhang , Wenyan Tang , Yue Zhang , Xin Lv , Yu-Ting Zhuang\",\"doi\":\"10.1016/j.jfca.2024.106971\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Rapid and precise detection of organophosphorus pesticides (OPs) is of great importance for life health. Herein, a new sensing platform is developed through a dual signal amplification approach for sensitive quantification of OPs, with a bimetallic metal organic framework (UiO-66(Zr/Fe)-NH<sub>2</sub>) as peroxide-like nanozyme and fluorescent indicator. In the presence of thiocholine (TCh), the hydrolyzate of acetylthiocholine iodide catalyzed by acetylcholinesterase, the catalytic oxidization of o-phenylenediamine by UiO-66(Fe/Zr)-NH<sub>2</sub> to strong fluorescent 2,3-diaminophenazine (λ<sub>em</sub> 555 nm) is significantly inhibited. OPs-triggered acetylcholinesterase inactivation enables the renaturation of UiO-66(Fe/Zr)-NH<sub>2</sub>. Interestingly, the introduction of 1,4-dioxane further boosts the amplification of fluorescence signals. By virtue of the recovered fluorescence at 555 nm and MOF fluorescence (λ<sub>em</sub> 446 nm), the dual signal amplification-based ratiometric fluorescent platform is successfully employed for the selective recognition and sensitive detection of OPs. This work is expected to provide a facile and sensitive method for accurate OPs assay, and make a positive contribution to food safety.</div></div>\",\"PeriodicalId\":15867,\"journal\":{\"name\":\"Journal of Food Composition and Analysis\",\"volume\":\"137 \",\"pages\":\"Article 106971\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-11-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Food Composition and Analysis\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0889157524010056\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Composition and Analysis","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0889157524010056","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
A ratiometric fluorescence platform based on bifunctional MOFs for sensitive detection of organophosphorus pesticides
Rapid and precise detection of organophosphorus pesticides (OPs) is of great importance for life health. Herein, a new sensing platform is developed through a dual signal amplification approach for sensitive quantification of OPs, with a bimetallic metal organic framework (UiO-66(Zr/Fe)-NH2) as peroxide-like nanozyme and fluorescent indicator. In the presence of thiocholine (TCh), the hydrolyzate of acetylthiocholine iodide catalyzed by acetylcholinesterase, the catalytic oxidization of o-phenylenediamine by UiO-66(Fe/Zr)-NH2 to strong fluorescent 2,3-diaminophenazine (λem 555 nm) is significantly inhibited. OPs-triggered acetylcholinesterase inactivation enables the renaturation of UiO-66(Fe/Zr)-NH2. Interestingly, the introduction of 1,4-dioxane further boosts the amplification of fluorescence signals. By virtue of the recovered fluorescence at 555 nm and MOF fluorescence (λem 446 nm), the dual signal amplification-based ratiometric fluorescent platform is successfully employed for the selective recognition and sensitive detection of OPs. This work is expected to provide a facile and sensitive method for accurate OPs assay, and make a positive contribution to food safety.
期刊介绍：
The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects.
The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.
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