POLD3 作为 DNA 复制修复的控制者

IF 5.6 2区生物学 International Journal of Molecular Sciences Pub Date : 2024-11-19 DOI:10.3390/ijms252212417

Nabilah Alli, Anna Lou-Hing, Edward L Bolt, Liu He

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引用次数: 0

摘要

多种 DNA 修复模式都需要 DNA 聚合酶进行 DNA 合成。真核生物 B-家族 DNA 聚合酶复合物 delta（Polδ）和 zeta（Polζ）在同源重组或单链 DNA 退火时帮助修复 DNA 链断裂。Polδ 和 Polζ 的 DNA 合成具有诱变性，但在 DNA 链断裂的情况下，细胞的存活也需要它们。Polδ和Polζ的POLD3亚基通过调节聚合酶功能和与其他DNA修复蛋白相互作用，是DNA重组修复的核心。我们介绍了发现 POLD3 的背景，研究了它的结构以及在细胞中的功能。我们着重介绍了 POLD3 尚未探索的结构方面和新的生化数据，这些数据将有助于理解 POLD3 在真核生物 DNA 修复和诱变中的关键作用，以及它对人类健康的影响。

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POLD3 as Controller of Replicative DNA Repair.

Multiple modes of DNA repair need DNA synthesis by DNA polymerase enzymes. The eukaryotic B-family DNA polymerase complexes delta (Polδ) and zeta (Polζ) help to repair DNA strand breaks when primed by homologous recombination or single-strand DNA annealing. DNA synthesis by Polδ and Polζ is mutagenic, but is needed for the survival of cells in the presence of DNA strand breaks. The POLD3 subunit of Polδ and Polζ is at the heart of DNA repair by recombination, by modulating polymerase functions and interacting with other DNA repair proteins. We provide the background to POLD3 discovery, investigate its structure, as well as function in cells. We highlight unexplored structural aspects of POLD3 and new biochemical data that will help to understand the pivotal role of POLD3 in DNA repair and mutagenesis in eukaryotes, and its impact on human health.

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来源期刊

International Journal of Molecular Sciences 化学-化学综合

自引率

10.70%

发文量

13472

审稿时长

1.7 months

期刊介绍： The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).