Melina Faggi, Cecilia Paparella, Patricia Perfumo, Juan Manuel Teijeiro
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DNA fragmentation, chromatin maturity, reactive oxygen species (ROS) levels, motility and protein phosphorylation levels analyses were addressed to each fraction.</p><p><strong>Results: </strong>The sperm DNA fragmentation was reduced in sperm recovered by swim-up in media with all concentrations of zinc assayed with respect to the control (p < 0.0001). Aniline blue staining showed better chromatin maturity in sperm recovered with 2.5- and 3.5-mM zinc (p = 0.045; p = 0.021). Kinematic parameters such as curvilinear velocity and beat-cross frequency showed improvement with 2.5 mM zinc (p = 0.0080 and p = 0.0400), whereas straightness, linearity, and hypermotility showed improvement with 5 mM zinc (p = 0.0075, p = 0.0069, and p = 0.0244). Protein phosphorylation patterns showed changes associated with treatment with zinc, and only 5 mM zinc treatment showed a decrease in ROS levels.</p><p><strong>Conclusion: </strong>The addition of zinc to mHTF provided optimal physiological conditions for sperm recovered through swim-up. 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引用次数: 0
摘要
目的：众所周知，锌会影响染色质的稳定性、活力和抗氧化能力。在辅助生殖技术（ART）中，游离精子仍是选择精子的首选方法，但与此过程相关的精子 DNA 断裂问题也引起了人们的关注。鉴于锌在保护精子 DNA 完整性和活力方面的重要作用，我们旨在研究蛙泳过程中补锌对精子质量的影响：方法：采用 203 名正常无精子症男性的精液样本。方法：使用 203 名正常无精子症男性的精液样本，将样本分成若干部分，并使用添加了三种不同浓度锌的人类输卵管液（mHTF）或不添加锌的培养基作为对照，进行上浮过程。对每个馏分进行DNA片段、染色质成熟度、活性氧（ROS）水平、运动能力和蛋白质磷酸化水平分析：结果：与对照组相比，在所有锌浓度的培养基中游动回收的精子 DNA 片段减少（p 结论）：在 mHTF 中添加锌可为通过游动回收的精子提供最佳生理条件。在选择用于 ART 的精子时应考虑添加锌。
Purpose: Zinc is known to influence chromatin stability, motility and protection against oxidative stress. While swim-up remains the preferred method for selecting sperm in Assisted Reproductive Technologies (ART), concerns arise regarding sperm DNA fragmentation associated with this procedure. Given zinc's significant role in protecting sperm DNA integrity and motility, we aimed to investigate the impact of zinc supplementation during the swim-up process on sperm quality.
Methods: Semen samples from 203 normozoospermic men were used. Samples were divided into fractions and swim-up procedure was applied using human tubal fluid (mHTF) supplemented with three different concentrations of zinc or medium without supplementation as control. DNA fragmentation, chromatin maturity, reactive oxygen species (ROS) levels, motility and protein phosphorylation levels analyses were addressed to each fraction.
Results: The sperm DNA fragmentation was reduced in sperm recovered by swim-up in media with all concentrations of zinc assayed with respect to the control (p < 0.0001). Aniline blue staining showed better chromatin maturity in sperm recovered with 2.5- and 3.5-mM zinc (p = 0.045; p = 0.021). Kinematic parameters such as curvilinear velocity and beat-cross frequency showed improvement with 2.5 mM zinc (p = 0.0080 and p = 0.0400), whereas straightness, linearity, and hypermotility showed improvement with 5 mM zinc (p = 0.0075, p = 0.0069, and p = 0.0244). Protein phosphorylation patterns showed changes associated with treatment with zinc, and only 5 mM zinc treatment showed a decrease in ROS levels.
Conclusion: The addition of zinc to mHTF provided optimal physiological conditions for sperm recovered through swim-up. This supplementation should be considered for selecting sperm for use in ART.
期刊介绍：
The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species.
The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.
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