通过低温电子断层扫描对神经元分子结构的新见解。

IF 4.8 2区 医学 Q1 NEUROSCIENCES Current Opinion in Neurobiology Pub Date : 2025-02-01 DOI:10.1016/j.conb.2024.102939
Arsen Petrovic , Thanh Thao Do , Rubén Fernández-Busnadiego
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引用次数: 0

摘要

低温电子断层扫描(cryo-ET)以分子分辨率显示原生保存的细胞超微结构。样品制备工作流程和图像处理工具的最新发展使cryo-ET在细胞神经生物学中的应用越来越广泛。因此,cryo-ET开始使用越来越复杂的样品来解开神经元的原位大分子组织。在这里,我们重点介绍了冷冻et技术的进展,并回顾了其最近在研究疾病条件下神经元结构及其变化方面的应用。
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New insights into the molecular architecture of neurons by cryo-electron tomography
Cryo-electron tomography (cryo-ET) visualizes natively preserved cellular ultrastructure at molecular resolution. Recent developments in sample preparation workflows and image processing tools enable growing applications of cryo-ET in cellular neurobiology. As such, cryo-ET is beginning to unravel the in situ macromolecular organization of neurons using samples of increasing complexity. Here, we highlight advances in cryo-ET technology and review its recent use to study neuronal architecture and its alterations under disease conditions.
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来源期刊
Current Opinion in Neurobiology
Current Opinion in Neurobiology 医学-神经科学
CiteScore
11.10
自引率
1.80%
发文量
130
审稿时长
4-8 weeks
期刊介绍: Current Opinion in Neurobiology publishes short annotated reviews by leading experts on recent developments in the field of neurobiology. These experts write short reviews describing recent discoveries in this field (in the past 2-5 years), as well as highlighting select individual papers of particular significance. The journal is thus an important resource allowing researchers and educators to quickly gain an overview and rich understanding of complex and current issues in the field of Neurobiology. The journal takes a unique and valuable approach in focusing each special issue around a topic of scientific and/or societal interest, and then bringing together leading international experts studying that topic, embracing diverse methodologies and perspectives. Journal Content: The journal consists of 6 issues per year, covering 8 recurring topics every other year in the following categories: -Neurobiology of Disease- Neurobiology of Behavior- Cellular Neuroscience- Systems Neuroscience- Developmental Neuroscience- Neurobiology of Learning and Plasticity- Molecular Neuroscience- Computational Neuroscience
期刊最新文献
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