微胶囊毛霉菌种的活力,作为优化生物防治用途的一种策略。

IF 2.1 4区 生物学 Q3 MICROBIOLOGY Brazilian Journal of Microbiology Pub Date : 2025-03-01 Epub Date: 2024-12-17 DOI:10.1007/s42770-024-01594-6
Thalesram Izidoro Pinotti, Tiago de Oliveira Sousa, Wéverson Lima Fonseca, Thiago Pajeú Nascimento, Helane França Silva, Romero Marcos Pedrosa Brandão Costa, Alice Maria Gonçalves Santos
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引用次数: 0

摘要

化学杀菌剂在没有有效控制植物病原体的情况下越来越多地使用,导致微生物产生耐药性。作为一种有前途的替代品,以木霉为原料的产品在综合疾病管理中具有可持续和有效的潜力。然而,主要的配方不提供对非生物因素的必要保护。本研究对海藻酸钠包封的木霉进行了贮藏活力实验,并对其拮抗潜能进行了研究。在生产后5、15、30、45、60和150天,分别在干燥或冷藏环境中进行电镀,评估胶囊的生存能力和储存条件。采用植物病原菌镰刀菌(Fusarium sp.)培养配对法测定其拮抗潜能。结果表明,在不同的储存环境下,可以保持分生孢子的活力。同时,该胶囊对被包被的分生孢子具有拮抗作用,对镰刀菌的生长有52.54%的抑制作用。因此,包封是一种替代配方方法,可确保分生木霉的生存能力,并优化其在生物防治中的应用。
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Viability of microencapsulated species of Trichoderma as a strategy to optimize use in biological control.

The increasing use of chemical fungicides without effective control of phytopathogens has led to the development of resistance in microorganisms. As a promising alternative, products formulated with Trichoderma have emerged for their sustainable and effective potential in integrated disease management. However, the predominant formulations do not offer the necessary protection against abiotic factors. In this study, we investigated Trichoderma species encapsulated in sodium alginate through storage viability experiments and their antagonistic potential. The viability and storage conditions of the capsules were evaluated by plating at 5, 15, 30, 45, 60, and 150 days after production, kept in dry or refrigerated environments. The antagonistic potential was determined by the culture pairing method using the phytopathogen Fusarium sp. The results demonstrated that it was possible to maintain the viability of the conidia, with no differences between storage environments. Additionally, the capsule provided UV protection to the conidia encapsulated species possess antagonistic potential, inhibiting 52.54% of Fusarium sp. growth. Consequently, encapsulation is an alternative formulation method that ensures the viability of Trichoderma conidia and optimizes its use in biological control.

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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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