一种用于人体汗液中尿酸检测的电化学装置的直接打印及其与纸的接口。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2025-01-20 Epub Date: 2025-01-09 DOI:10.1021/acsabm.4c01706
Rutuja Kale, Mayur Krishna Das, Arun Dodde Gowda, Sagar A Raut, Jasirali Pannikkandathil, Saurabh Bodake, Roshan M Borkar, Suman Pahal, Saurabh Kumar
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引用次数: 0

摘要

利用激光刻写(直接打印)技术,我们在柔性聚酰亚胺片上制造了一种无酶、无介质、纸界面的尿酸检测电化学装置(P-LSG)。通过对各种纸基材料的研究,发现Whatman滤纸-1在电解液体积较小(20 μL)的情况下能获得最佳的电化学信号。此外,用纳米金修饰Whatman滤纸1,以提高P-LSG器件的电催化活性。制备的aunp修饰的P-LSG生物传感器在10 ~ 750 μM范围内具有良好的尿酸检测电催化活性,灵敏度为~ 0.214 μA μM-1 cm-2,检测限为~ 1.4 μM。采用UHPLC-ESI-MS/MS技术对传感器进行验证,检测回收率小于10%。这项工作为用各种纳米材料修饰纸基板以改善传感参数打开了窗口。此外,纸张的电解质储存能力和丰富的化学性质为生物识别元件诊断其他代谢物提供了有效的固定化平台。此外,它有潜力利用纸张分析小样本(如汗液、眼泪、尿液等)的体积,以开发无创可穿戴生物传感器。
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Direct Printing of an Electrochemical Device and Its Interface with Paper for Uric Acid Detection in Human Sweat.

Using a laser-scribed (direct printing) technique, we have fabricated an enzymeless, mediatorless, and paper-interfaced electrochemical device (P-LSG) for uric acid detection on a flexible polyimide sheet. Various paper substrates were investigated, and it was found that Whatman filter paper-1 is promising to obtain the best electrochemical signals at the small volume of electrolyte, i.e., 20 μL. Furthermore, the Whatman filter paper-1 was modified with gold nanoparticles (AuNPs) to improve the electrocatalytic activity of the P-LSG device. The fabricated AuNP-modified P-LSG biosensor exhibited excellent electrocatalytic activity for the detection of uric acid over a wide range of 10 to 750 μM, with sensitivity of ∼0.214 μA μM-1 cm-2, and a limit of detection of ∼1.4 μM. The sensor was further validated by using the UHPLC-ESI-MS/MS technique, and the observed percentage recovery was less than 10%. This work opens the window to modified paper substrates with various nanomaterials to improve the sensing parameters. The electrolyte storage capacity and rich chemistry of paper additionally provide an efficient immobilization platform for biorecognition elements to diagnose other metabolites. Furthermore, it has the potential to analyze the volume of small samples (like sweat, tears, urine, etc.) using paper to develop noninvasive wearable biosensors.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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