Yingxia Guo , Meichen Liu , Jiye Tian , Chunpeng Feng , Qingbin Wang , Xuan Zhao , Lei Yin
{"title":"UHPLC-MS/MS法研究mPEG5-NH2聚合物在大鼠体内的药动学行为。","authors":"Yingxia Guo , Meichen Liu , Jiye Tian , Chunpeng Feng , Qingbin Wang , Xuan Zhao , Lei Yin","doi":"10.1016/j.jchromb.2025.124460","DOIUrl":null,"url":null,"abstract":"<div><div>As an important chemical reagent, methoxy polyethylene glycol amine (mPEG-NH<sub>2</sub>) is widely used in biomedical field. Unraveling the pharmacokinetic behavior of mPEG-NH<sub>2</sub> polymers is essential for revealing the toxicity and efficiency of mPEG-NH<sub>2</sub> related drug delivery systems. In this study, a simple analytical assay based on mass spectrometry (MS) was first established and validated for quantification of mPEG<sub>5</sub>-NH<sub>2</sub> in biological matrix. The multiple reaction monitoring (MRM) transitions at <em>m</em>/<em>z</em> 252.2 (precursor ions) → 87.7 (fragment ions) and <em>m</em>/<em>z</em> 371.2 (precursor ions) → 89.2 (fragment ions) were chosen to determine mPEG<sub>5</sub>-NH<sub>2</sub> and OH-PEG<sub>8</sub>-OH, respectively. The UHPLC-MS/MS assay showed excellent linearity over the range of 0.01–10 μg/mL. Intra-day and inter-day accuracies and precisions of the assay were all within ± 6.44 %. The analytical assay was successfully applied to reveal the <em>in vivo</em> pharmacokinetic behavior of mPEG<sub>5</sub>-NH<sub>2</sub> in rats.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1252 ","pages":"Article 124460"},"PeriodicalIF":2.8000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unraveling the in vivo pharmacokinetic behavior of mPEG5-NH2 polymer in rats by UHPLC-MS/MS assay\",\"authors\":\"Yingxia Guo , Meichen Liu , Jiye Tian , Chunpeng Feng , Qingbin Wang , Xuan Zhao , Lei Yin\",\"doi\":\"10.1016/j.jchromb.2025.124460\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>As an important chemical reagent, methoxy polyethylene glycol amine (mPEG-NH<sub>2</sub>) is widely used in biomedical field. Unraveling the pharmacokinetic behavior of mPEG-NH<sub>2</sub> polymers is essential for revealing the toxicity and efficiency of mPEG-NH<sub>2</sub> related drug delivery systems. In this study, a simple analytical assay based on mass spectrometry (MS) was first established and validated for quantification of mPEG<sub>5</sub>-NH<sub>2</sub> in biological matrix. The multiple reaction monitoring (MRM) transitions at <em>m</em>/<em>z</em> 252.2 (precursor ions) → 87.7 (fragment ions) and <em>m</em>/<em>z</em> 371.2 (precursor ions) → 89.2 (fragment ions) were chosen to determine mPEG<sub>5</sub>-NH<sub>2</sub> and OH-PEG<sub>8</sub>-OH, respectively. The UHPLC-MS/MS assay showed excellent linearity over the range of 0.01–10 μg/mL. Intra-day and inter-day accuracies and precisions of the assay were all within ± 6.44 %. The analytical assay was successfully applied to reveal the <em>in vivo</em> pharmacokinetic behavior of mPEG<sub>5</sub>-NH<sub>2</sub> in rats.</div></div>\",\"PeriodicalId\":348,\"journal\":{\"name\":\"Journal of Chromatography B\",\"volume\":\"1252 \",\"pages\":\"Article 124460\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Chromatography B\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1570023225000121\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/10 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chromatography B","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1570023225000121","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/10 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Unraveling the in vivo pharmacokinetic behavior of mPEG5-NH2 polymer in rats by UHPLC-MS/MS assay
As an important chemical reagent, methoxy polyethylene glycol amine (mPEG-NH2) is widely used in biomedical field. Unraveling the pharmacokinetic behavior of mPEG-NH2 polymers is essential for revealing the toxicity and efficiency of mPEG-NH2 related drug delivery systems. In this study, a simple analytical assay based on mass spectrometry (MS) was first established and validated for quantification of mPEG5-NH2 in biological matrix. The multiple reaction monitoring (MRM) transitions at m/z 252.2 (precursor ions) → 87.7 (fragment ions) and m/z 371.2 (precursor ions) → 89.2 (fragment ions) were chosen to determine mPEG5-NH2 and OH-PEG8-OH, respectively. The UHPLC-MS/MS assay showed excellent linearity over the range of 0.01–10 μg/mL. Intra-day and inter-day accuracies and precisions of the assay were all within ± 6.44 %. The analytical assay was successfully applied to reveal the in vivo pharmacokinetic behavior of mPEG5-NH2 in rats.
期刊介绍：
The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis.
Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches.
Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.
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