肠沙门氏菌血清型中ttrA和pduA基因的缺失在不同鸡系中诱导了对野生型感染的相似免疫反应。

IF 2.5 2区农林科学 Q1 VETERINARY SCIENCES Avian Pathology Pub Date : 2025-02-05 DOI:10.1080/03079457.2025.2457095

Túlio Spina de Lima, Mauro de Mesquita Souza Saraiva, Adriana Maria de Almeida, Taisa Santiago Ferreira, Lucas Bocchini Rodrigues Alves, Viviane Amorim Ferreira, Oliveiro Caetano de Freitas Neto, Paul Andrew Barrow, Angelo Berchieri Junior

引用次数: 0

摘要

此前有报道称，沙门氏菌通过ttr和pdu操纵子编码的代谢途径对四硫酸盐和1,2-丙二醇的利用与厌氧环境中微生物群的过度生长和竞争有关。然而，最近的研究表明，在缺乏ttr和pdu基因的菌株中，与各自的野生型菌株相比，哪些菌株在肠道内定植和在粪便上传播细菌的数量更高，并且产生更突出的炎症。本研究对ttrA和pduA基因缺失的鼠伤寒沙门菌（STM）和肠炎沙门菌（SE）感染不同世系雏鸡的免疫应答进行了研究。我们的工作分为两个实验，每个菌株一个，利用108只鸡收集脾脏和盲肠扁桃体，通过RT-qPCR检测免疫反应。从免疫反应分析来看，肠炎沙门氏菌突变型和野生型菌株在感染后第一天引发了上调的促炎细胞因子，与鼠伤寒沙门氏菌菌株（突变型和野生型）相比，情况正好相反。然而，这些缺失并没有损害与盲肠扁桃体和脾脏中各自野生型相关的突变株产生的免疫应答，这表明这些代谢途径对定植成功不是必需的。综上所述，在缺乏ttrA和pduA基因的情况下，SE和STM引起的免疫反应强度与各自的野生型菌株相同。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Deletion of ttrA and pduA genes in Salmonella enterica serovars induce a comparable immune response to wild-type infection in different chicken lineages.

Research highlights: ttrA and pduA double mutants in Salmonella provoke a similar immune response.SE elicited more intense immune responses than STM.The immune response in the broiler was more intense than in other lineages.

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来源期刊

Avian Pathology 农林科学-兽医学

CiteScore

4.50

自引率

10.70%

发文量

审稿时长

1 months

期刊介绍： Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.