Identification of Primary Metabolites in 1H NMR-Based Metabolomics of Plants.

Identifying metabolites in NMR-based plant metabolomics is challenging due to the complexity of plant metabolites. This complexity stems from the abundance and diverse chemical properties of compounds, which vary in concentration across plant specimens. Additionally, the lack of automated identification software complicates the analysis process. Primary metabolites such as amino acids and sugars are widespread in plants, yet their identification is not straightforward due to various stereoisomeric forms and dynamic equilibria. Our protocol offers a manual approach to identify these metabolites, particularly focusing on amino acids and sugars. Through step-by-step guidance, we aim to empower researchers to navigate plant metabolomics complexities effectively.