Shining a light on cell biology of the nucleus with single-cell sequencing

From the preservation of genomic integrity to the regulation of RNA translation, nearly all cellular processes are regulated in a cell context-dependent manner. To fully understand the context-specific function of involved nuclear processes, a vast number of single-cell sequencing technologies were developed over the last decade. This instrumental work demonstrated the heterogeneity between cell types and individual cells, bringing about new understanding of nuclear mechanisms and their crosstalk to cell states. In this review, we will cover new technological advances and their exciting applications as well as future opportunities to discover new nuclear processes and the crosstalk between them.