角鲨烯单加氧酶对胆固醇的感应

Q4 Biochemistry, Genetics and Molecular Biology Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4284-9_12

Hudson W Coates, Andrew J Brown

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Sensing of Cholesterol by Squalene Monooxygenase.

Feedback regulation of squalene monooxygenase (SM), a rate-limiting enzyme of cholesterol synthesis, is an important component of cellular lipid homeostasis. This regulation is exerted in part by the cholesterol-induced degradation of the SM protein. Here, we describe the cell culture, sample preparation, and immunoblotting conditions that our laboratory routinely uses to study the cholesterol regulation of both endogenous and ectopic SM. We also provide a worked example of quantifying the cholesterol-induced degradation of SM using densitometry.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.