Domenica G De Luca, Xiao Rui Li, David C Alexander, Tanis C Dingle, Philippe J Dufresne, Linda M Hoang, Julianne V Kus, Caroline Sheitoyan-Pesant, Amrita Bharat
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Comparison of broth microdilution and Etest® methods for susceptibility testing of amphotericin B in Candida auris.
Amphotericin B remains an important treatment for multidrug-resistant Candida auris. Antifungal susceptibility testing of amphotericin B in C. auris can vary depending on the methodology used. Here, we compared the Etest method and the Clinical and Laboratory Standards Institute broth microdilution reference method for amphotericin B against 60 clinical C. auris isolates from the four major clades. The minimum inhibitory concentrations differed significantly by method (P-value, <.0001), and discrepancies were observed in the interpretation of resistance (categorical agreement, 88.3%; very major error, 33.3%). Broth microdilution may represent a more conservative approach for detecting amphotericin B resistance in C. auris.
期刊介绍：
Medical Mycology is a peer-reviewed international journal that focuses on original and innovative basic and applied studies, as well as learned reviews on all aspects of medical, veterinary and environmental mycology as related to disease. The objective is to present the highest quality scientific reports from throughout the world on divergent topics. These topics include the phylogeny of fungal pathogens, epidemiology and public health mycology themes, new approaches in the diagnosis and treatment of mycoses including clinical trials and guidelines, pharmacology and antifungal susceptibilities, changes in taxonomy, description of new or unusual fungi associated with human or animal disease, immunology of fungal infections, vaccinology for prevention of fungal infections, pathogenesis and virulence, and the molecular biology of pathogenic fungi in vitro and in vivo, including genomics, transcriptomics, metabolomics, and proteomics. Case reports are no longer accepted. In addition, studies of natural products showing inhibitory activity against pathogenic fungi are not accepted without chemical characterization and identification of the compounds responsible for the inhibitory activity.
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